PATL2 regulates mRNA homeostasis in oocytes by interacting with EIF4E and CPEB1
PATL2 regulates mRNA homeostasis in oocytes by interacting with EIF4E and CPEB1
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DOI：
10.1242/dev.201572
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Lei Wang
中科院分区：
文献类型：
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作者：
Zhihua Zhang;Ruyi Liu;Hongbin Zhou;Qun Li;Ronggui Qu;Weijie Wang;Zhou Zhou;Ran Yu;Yang Zeng;Jian Mu;Biaobang Chen;Xuejiang Guo;Qing Sang;Lei Wang
The accumulation and storage of maternal mRNA is crucial for oocyte maturation and embryonic development. PATL2 is an oocyte-specific RNA-binding protein, and previous studies have confirmed that PATL2 mutation in Human and knockout mice cause oocyte maturation arrest or embryonic development arrest, respectively. However, the physiological function of PATL2 in the process of oocyte maturation and embryonic development is largely unknown. Here, we report that PATL2 is highly expressed in growing oocytes and coupled with EIF4E and CPEB1 to regulate maternal mRNA expression in immature oocytes. The GV oocytes from Patl2−/- mice exhibit decreasing maternal mRNA expression and reduced levels of protein synthesis. We further confirmed PATL2 phosphorylation in the oocyte maturation process and identified the S279 phosphorylation site using phosphoproteomics. We found the S279D mutation decreased the protein level of PATL2 and led to subfertility in Palt2S279D knock-in mice. Our work reveals the previously unrecognized role of PATL2 in regulating the maternal transcriptome and shows that phosphorylation of PATL2 leads to the regulation of PATL2 protein levels via ubiquitin-mediated proteasomal degradation in oocytes.
