Characterization of DNA methylation variations during fruit development and ripening of Vitis vinifera (cv. 'Fujiminori')
Characterization of DNA methylation variations during fruit development and ripening of Vitis vinifera (cv. 'Fujiminori')
复制标题
DOI：
10.1007/s12298-020-00759-5
复制
发表时间：
2020
影响因子：
3.5
通讯作者：
Fang Jinggui
中科院分区：
文献类型：
--
作者：
Shangguan Lingfei;Fang Xiang;Jia Haifeng;Chen Mengxia;Zhang Kekun;Fang Jinggui
葡萄果实是葡萄最重要的经济器官，为了研究葡萄藤基因组甲基化水平及其在果实发育中的功能意义，我们构建了葡萄藤果实不同发育阶段的全基因组DNA甲基化图谱。本研究对13个DNA甲基化相关基因及其表达谱进行了鉴定和分析。mC、mCG、mCHG和mCHH甲基化水平在开花后65天（65天）果实（véraison期）高于40天（绿色期）和90天（成熟期）果实。相对于mC背景下的甲基化，mCHH背景下的甲基化水平高于mCG和mCHG。ncRNA区域的DNA甲基化水平显著高于外显子、基因、内含子和mRNA区域。65 DAF_vs_40 cDNA的差异甲基化区域（DMR）和差异甲基化启动子（DMPs）均高于90 DAF_vs_65 cDNA和90 DAF_vs_40 cDNA。大多数DMR（或DMP）参与代谢过程和细胞过程，结合和催化活性。这些结果表明，葡萄果实发育过程中DNA甲基化抑制基因表达，这拓宽了我们对葡萄藤基因组中DNA甲基化的景观和功能的理解。
The fruit is the most important economical organ in the grape; accordingly, to investigate the grapevine genomic methylation landscape and examine its functional significance during fruit development, we generated whole genome DNA methylation maps for various developmental stages in the fruit of grapevine. In this study, thirteen DNA methylation-related genes and their expression profiles were identified and analyzed. The methylation levels for mC, mCG, mCHG, and mCHH contexts in 65 days after flowering (65DAF) fruit (véraison stage) were higher than those in 40DAF (green stage) and 90DAF (mature stage) fruits. Relative to methylation in the mC context, methylation levels in the mCHH context were higher than those of mCG and mCHG. The DNA methylation level in the ncRNA regions was significantly higher than that in exon, gene, intron, and mRNA regions. The differentially methylated regions (DMRs) and differentially methylated promoters (DMPs) in 65DAF_vs_40DAF were both higher than those in 90DAF_vs_65DAF and 90DAF_vs_40DAF. Most DMRs (or DMPs) were involved in metabolic processes and cell processes, binding, and catalytic activity. These results indicated that DNA methylation represses gene expression during grape fruit development, and it broadens our understanding of the landscape and function of DNA methylation in grapevine genomes.