The separation of myo-inositol phosphates by ion-pair chromatography.
The separation of myo-inositol phosphates by ion-pair chromatography.
复制标题
通过离子对色谱法分离肌醇磷酸盐。
DOI：
10.1016/0006-291x(88)90566-9
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发表时间：
1988
影响因子：
3.1
通讯作者：
BeMent,DM
中科院分区：
文献类型：
--
作者：
Shayman,JA;BeMent,DM
研究了离子对反相高效液相色谱分离肌醇磷酸酯的方法。肌醇磷酸盐的保留率取决于所用杂多酸的极性和溶剂的pH值。提出了一种方法，该方法允许等度分离多种形式的肌醇磷酸，包括肌醇三磷酸的异构体。这种方法似乎优于目前使用的基于阴离子交换的系统，因为保留体积较小，所用溶剂的离子强度较低，不需要重新平衡，并且能够等比例地进行分离。
The separation of myo-inositol phosphates by ion pair, reverse-phase high performance liquid chromatography has been investigated. The retention of the inositol phosphates is dependent on both the polarity of the hetaeron utilized and on the pH of the solvent. A method is presented which permits the isocratic separation of multiple forms of inositol phosphates including isomers of myo-inositol trisphosphate. This method appears to be superior to the anion exchange based systems currently employed because of smaller retention volumes, the low ionic strength of the solvent employed, the absence of a requirement for reequilibration, and the ability to perform separations isocratically.