Development of a sensitive ELISA for the determination of fumonisin B1 in cereals
Development of a sensitive ELISA for the determination of fumonisin B1 in cereals
复制标题
DOI：
10.1021/jf990731m
复制
发表时间：
2000-07-01
影响因子：
6.1
通讯作者：
Solti, L
中科院分区：
文献类型：
--
作者：
Barna-Vetró, I;Szabó, E;Solti, L
用短交联剂（戊二醛）制备的FB 1-戊二醛-dekeyhole血蓝蛋白免疫原，制备了高效价的伏马菌素B-1单克隆抗体。所选单克隆抗体对FB 1、FB 2和FB 3的平均交叉反应性分别为100、91.8和209%;未发现与水解伏马菌素的反应性。用该抗体建立了一种直接的、竞争性的酶联免疫吸附测定法，用于谷物中FB 1的定量测定。采用50%乙腈基溶剂加添加剂对谷物进行浸提，浸提液稀释后不经净化直接使用。标准曲线的平均批内和批间变异系数为
Monoclonal fumonisin B-1 antibodies with high titer were raised by using FB1-glutaraldehydekeyhole limpet hemocyanin immunogen prepared by a short cross-linker reagent (glutaraldehyde). Mean cross-reactivities of the selected monoclonal antibody for FB1, FB2, and FB3 were 100, 91.8, and 209%, respectively; no reactivity was found with hydrolyzed fumonisin. A direct, competitive enzyme-linked immunosorbent assay for the quantitative determination of FB1 in cereals has been developed with this antibody. Fifty percent acetonitrile-based solvent with some additives was used for extraction of cereals, and the diluted extracts were used without cleanup in the test. The mean within-assay and interassay coefficients of variation for the standard curve were