Single-molecule labeling for studying trafficking of renal transporters.
Single-molecule labeling for studying trafficking of renal transporters.
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用于研究肾脏转运蛋白运输的单分子标记。
DOI：
10.1152/ajprenal.00082.2017
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ortiz,PabloA
中科院分区：
文献类型：
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作者：
Jaykumar,AnkitaBachhawat;Caceres,PauloS;Ortiz,PabloA
检测和跟踪单分子的能力体现了以时间和空间分辨率可视化跨膜蛋白质的复杂行为的优势，否则传统的标记和生化技术将失去这种分辨率。新型成像探头的发展为研究它们的传输和表面动力学提供了一种可靠的方法。这篇简短的综述集中于目前可用于跨膜蛋白的单分子标记的技术，它们的优点和局限性。我们还结合最近的研究讨论了这些技术在肾转运研究中的应用。
The ability to detect and track single molecules presents the advantage of visualizing the complex behavior of transmembrane proteins with a time and space resolution that would otherwise be lost with traditional labeling and biochemical techniques. Development of new imaging probes has provided a robust method to study their trafficking and surface dynamics. This mini-review focuses on the current technology available for single-molecule labeling of transmembrane proteins, their advantages, and limitations. We also discuss the application of these techniques to the study of renal transporter trafficking in light of recent research.