CRISPR provides acquired resistance against viruses in prokaryotes
CRISPR provides acquired resistance against viruses in prokaryotes
复制标题
DOI：
10.1126/science.1138140
复制
发表时间：
2007-03-23
期刊：
影响因子：
56.9
通讯作者：
Horvath, Philippe
中科院分区：
文献类型：
--
作者：
Barrangou, Rodolphe;Fremaux, Christophe;Horvath, Philippe
规则间隔短回文重复序列（CRISPR）是大多数细菌和细菌基因组的显著特征，被认为与对噬菌体的抗性有关。我们发现，在病毒攻击后，细菌整合了来自噬菌体基因组序列的新间隔区。去除或添加特定间隔区修饰了细胞的噬菌体抗性表型。因此，CRISPR与相关的cas基因一起提供了对cas的抗性，并且抗性特异性由间隔子-噬菌体序列相似性决定。
Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.