Manganese peroxidases, laccases and oxalic acid from the selective white-rot fungus Physisporinus rivulosus grown on spruce wood chips
Manganese peroxidases, laccases and oxalic acid from the selective white-rot fungus Physisporinus rivulosus grown on spruce wood chips
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DOI：
10.1016/j.enzmictec.2004.10.004
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发表时间：
2005-03-02
影响因子：
3.4
通讯作者：
Hatakka, A
中科院分区：
文献类型：
--
作者：
Hakala, TK;Lundell, T;Hatakka, A
白腐菌Physisporinus rivulosus T241i在云杉（Picea abies）木屑上生长时产生锰过氧化物酶（MnP）、漆酶和草酸。该白腐担子菌可选择性降解木质素，在软木生物制浆方面具有广阔的应用前景。在木屑固态培养过程中，真菌产生草酸（28 μ mol/g干木材）和高MnP活性（24 μ mol/g干木材），而漆酶活性可忽略不计。用阴离子交换色谱法对预纯化的漆酶和MnP异构体进行分离。分离的MnP组分的表征表明，存在几个MnP异构体，pI值在3.4 ~ 3.9之间，分子质量在47 ~ 52 kDa之间。4种漆酶同工型的pI值在3.1 ~ 3.3之间，分子量在66 ~ 68 kDa之间。在4周的培养过程中，MnP和漆酶异构体没有变化。4种漆酶和2种MnP同工酶的n端氨基酸序列与另外两种用于生物造浆的选择性白腐菌Ceriporiopsis subvermispora CZ-3和IZU-154相似。(C) 2004爱思唯尔公司版权所有。
The white-rot fungus Physisporinus rivulosus T241i produced manganese peroxidase (MnP), laccase, and oxalic acid when it was grown on spruce (Picea abies) wood chips. This white-rot basidiomycete degrades lignin selectively and is promising for use in biopulping of softwood. During solid-state cultivation on wood chips the fungus produced oxalic acid (28 mumol/g dry wood) and a high MnP activity (24 nkat/g dry wood), while measured laccase activity was negligible. The prepurified laccase and MnP isoforms were fractionated with anion exchange chromatography. Characterization of the isolated MnP fractions indicated the presence of several MnP isoforms with pI values between 3.4 and 3.9 and molecular masses between 47 and 52 kDa. The four laccase isoforms had pI values between 3.1 and 3.3 and molecular masses between 66 and 68 kDa. The MnP and laccase isoform profiles did not vary during the 4-week cultivation. The N-terminal amino acid sequences of four laccase and two MnP isoenzymes resembled those of the two other selective white-rot fungi applied in biopulping, i.e. Ceriporiopsis subvermispora CZ-3 and the strain IZU-154. (C) 2004 Elsevier Inc. All rights reserved.