Fast, sensitive and cost-effective detection of nerve agents in the gas phase using a portable instrument and an electrochemical biosensor
Fast, sensitive and cost-effective detection of nerve agents in the gas phase using a portable instrument and an electrochemical biosensor
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DOI：
10.1007/s00216-007-1330-z
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发表时间：
2007-07-01
影响因子：
4.3
通讯作者：
Palleschi, Giuseppe
中科院分区：
文献类型：
--
作者：
Arduini, Fabiana;Amine, Aziz;Palleschi, Giuseppe
神经毒剂是已知在恐怖袭击中使用的化学战剂。由于可能的恐怖主义袭击的持续威胁，第一反应人员和军事人员使用的廉价和便携式系统引起了人们的兴趣。基于胆碱酯酶抑制的安培生物传感器显示出这种潜力。本研究将丁基胆碱酯酶固定在普鲁士蓝修饰的丝网印刷电极上，通过测定酶的残留活性进行神经毒剂检测。优化后的生物传感器在沙林和VX标准溶液中进行检测，检测限分别为12和14 ppb（抑制率的10%）。将生物传感器暴露在气相沙林中也获得了酶抑制作用。在不同的孵育时间（从30秒到10分钟）下测试了两种不同浓度的沙林气体（0.1和0.5 mg m(-3)）。在30秒的孵育时间内，可以检测到浓度为0.1 mg m（-3）的沙林，抑制度为34%，符合法定限度（对生命和健康有直接危险）。
The nerve agents are chemical warfare agents known to be used during terrorist attacks. An inexpensive and portable system to be used by first responders and military personnel is of interest owing to the continuing threat of possible terrorist attacks. Amperometric biosensors based on cholinesterase inhibition show such potentialities. In this work butyrylcholinesterase was immobilized onto screen-printed electrodes modified with Prussian blue and the nerve agent detection was performed by measuring the residual activity of enzyme. The optimized biosensor was tested with sarin and VX standard solutions, showing detection limits of 12 and 14 ppb (10% of inhibition), respectively. The enzymatic inhibition was also obtained by exposing the biosensors to sarin in gas phase. Two different concentrations of sarin gas (0.1 and 0.5 mg m(-3)) at different incubation times (from 30 s up to 10 min) were tested. It is possible to detect sarin at a concentration of 0.1 mg m(-3) with 30-s incubation time, with a degree of inhibition of 34%, which match the legal limits (immediate danger to life and health).