CHARACTERIZATION OF HERPES-SIMPLEX VIRUS TYPE-2 LATENCY-ASSOCIATED TRANSCRIPTION IN HUMAN SACRAL GANGLIA AND IN CELL-CULTURE
CHARACTERIZATION OF HERPES-SIMPLEX VIRUS TYPE-2 LATENCY-ASSOCIATED TRANSCRIPTION IN HUMAN SACRAL GANGLIA AND IN CELL-CULTURE
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DOI：
10.1093/infdis/163.1.23
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发表时间：
1991-01-01
影响因子：
6.4
通讯作者：
STRAUS, SE
中科院分区：
文献类型：
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作者：
CROEN, KD;OSTROVE, JM;STRAUS, SE
单纯疱疹病毒2型（HSV-2）在骶背根感觉神经节中潜伏并重新激活的能力是复发性生殖器肝炎的基础。采用原位杂交技术研究了HSV-2基因在潜伏感染的人骶神经节中的表达。用HSV-2长重复区探针杂交发现，9个个体中有5个的骶神经节神经元上有强烈的核信号。检测到的RNA与受感染细胞蛋白O的转录本重叠，但方向相反，或“反义”。这些观察结果与先前在人类三叉神经节中对HSV-1的观察结果相似，并证实了最近在hsv -2感染小鼠和豚鼠潜伏期中的发现。来自受感染的Vero细胞的RNA北杂交显示，HSV-2潜伏期相关转录物的大小与HSV-1潜伏期转录物的大小相似（1.85 kb）。因此，HSV-1和HSV-2在人类感觉神经节中的潜伏期即使不完全相同，在细胞定位和转录模式方面也是相似的。
The ability of herpes simplex virus type 2 (HSV-2) to establish latency in and reactivate from sacral dorsal root sensory ganglia is the basis for recurrent genital hepes. The expression of HSV-2 genes in latently infected human sacral ganglia was investigated by in situ hybridization. Hybridizations with a probe from the long repeat region of HSV-2 revealed strong nuclear signals overlying neurons in sacral ganglia from five of nine individuals. The RNA detected overlaps with the transcript for infected cell protein O but in the opposite, or "anti-sense," orientation. These observations mimic those made previously with HSV-1 in human trigeminal ganglia and confirm the recent findings during latency in HSV-2-infected mice and guinea pigs. Northern hybridization of RNA from infected Vero cells showed that an HSV-2 latency-associated transcript was similar in size to the larger (1.85 kb) latency transcript of HSV-1. Thus, HSV-1 and HSV-2 latency in human sensory ganglia are similar, if not identical, in terms of their cellular localization and pattern of transcription.