Selective production of rubusoside from stevioside by using the sophorose activity of beta-glucosidase from Streptomyces sp GXT6
Selective production of rubusoside from stevioside by using the sophorose activity of beta-glucosidase from Streptomyces sp GXT6
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利用链霉菌 GXT6 β-葡萄糖苷酶的槐糖活性从甜菊苷中选择性生产甜叶悬钩子苷
DOI：
10.1007/s00253-015-6802-z
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发表时间：
2015
影响因子：
5
通讯作者：
Liqin Du
中科院分区：
文献类型：
--
作者：
Zilong Wang;Jinpei Wang;Minhua Jiang;Yutuo Wei;Hao Pang;Hang Wei;Ribo Huang;Liqin Du
为了生产甜菊糖苷，研究了对甜菊糖苷具有优先特异性的酶从甜菊糖苷中生产甜菊糖苷的能力。我们从链霉菌中鉴定出一种β-葡萄糖苷酶BGL1。GXT6，作为一种生产鲁布糖苷的酶。在几种糖底物中，BGL1对槐糖的亲和力最强。这种酶只水解甜菊苷中C-13的苦参苷的葡萄糖部分。采用核磁共振（NMR）和核磁共振（1h）测定了冬冬苷的产率。以甜菊糖苷为原料制备甜菊糖苷，甜菊糖苷转化率为98.2%。在6 h内，野樱草苷的产率为78.8%。
In order to produce rubusoside, enzymes with preferential specificity for the saccharide sophorose were tested for ability to produce rubusoside from stevioside. We identified BGL1, a β-glucosidase fromStreptomycessp. GXT6, as an enzyme for rubusoside production. Out of several saccharide substrates, BGL1 showed the most affinity to sophorose. This enzyme only hydrolyzes the glucose moiety of the sophoroside at C-13 in stevioside. Production of rubusoside was determined by1H and13C nuclear magnetic resonance (NMR). Thus, rubusoside was produced from stevioside and the stevioside conversion rate was 98.2 %. The production yield of rubusoside was 78.8 % in 6 h.