A novel monopartite dsRNA virus isolated from the entomopathogenic and nematophagous fungus Purpureocillium lilacinum
A novel monopartite dsRNA virus isolated from the entomopathogenic and nematophagous fungus Purpureocillium lilacinum
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DOI：
10.1007/s00705-016-3045-y
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发表时间：
2016-12-01
影响因子：
2.7
通讯作者：
Herrero, Noemi
中科院分区：
文献类型：
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作者：
Herrero, Noemi
淡紫紫孢菌是一种普遍存在的腐殖真菌，通常从土壤中分离，并被广泛称为植物病原线虫和害虫的生物防治剂。真菌病毒感染许多真菌物种，但病毒感染昆虫病原真菌的研究仍然是最近。在这项研究中，分析了从捷克共和国各地自然和栽培栖息地的土壤中收集的总共86个紫丁香假单胞菌分离株; 22%的分离株含有具有病毒特征的双链RNA（dsRNA）元件。这些结果表明，真菌病毒是常见的淡紫青霉。在调查中检测到的最常见的dsRNA元件之一被完全测序，并对应于以前未描述的真菌病毒的2，864-bp基因组，命名为Purpureocillium lilacinum nonsegmented virus 1（PlNV-1）。系统发育分析表明，该病毒可能属于一个新的分类单位，与部分病毒科有关。
Purpureocillium lilacinum is a ubiquitous saprophytic fungus commonly isolated from soils and widely known as a biological control agent against phytopathogenic nematodes and pest insects. Mycoviruses infect a wide number of fungal species, but the study of viruses infecting entomopathogenic fungi is still quite recent. In this study, a total of 86 P. lilacinum isolates collected from soil in natural and cultivated habitats throughout the Czech Republic were analyzed; 22 % of the isolates harbored double-stranded RNA (dsRNA) elements with viral characteristics. These results suggest that mycoviruses are common in P. lilacinum. One of the most common dsRNA elements detected in the survey was completely sequenced and corresponded to the 2,864-bp genome of a previously undescribed mycovirus, designated Purpureocillium lilacinum nonsegmented virus 1 (PlNV-1). Phylogenetic analysis of the RNA-dependent RNA polymerase of PlNV-1 indicated that this virus might belong to a new taxon related to the family Partitiviridae.