The Anti-Oxidant Ergothioneine Augments the Immunomodulatory Function of TLR Agonists by Direct Action on Macrophages.
The Anti-Oxidant Ergothioneine Augments the Immunomodulatory Function of TLR Agonists by Direct Action on Macrophages.
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抗氧化剂甲氨酸通过直接对巨噬细胞作用的直接作用来增强TLR激动剂的免疫调节功能。
DOI：
10.1371/journal.pone.0169360
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Seya T
中科院分区：
文献类型：
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作者：
Yoshida S;Shime H;Funami K;Takaki H;Matsumoto M;Kasahara M;Seya T
L-麦角硫因（EGT）是一种天然存在的氨基酸，其特征在于其抗氧化特性;然而，EGT的生理作用尚未确定。我们研究了EGT的免疫增强特性，发现它作为Toll样受体（TLR）信号传导的增强剂。当小鼠骨髓源性巨噬细胞（BMDM）与EGT预处理，TLR信号介导的细胞因子的生产增加BMDM。结果与TLR 2，3，4和7激动剂是可重复的。特别是，IL-6和IL-12 p40进一步升高，通过预处理与EGT在BMDM中，提示诱导M1极化。在与OT-II CD 4 + T细胞和脾F4/80+巨噬细胞的共培养试验中，EGT显著诱导CD 4 + T细胞中的Th 17偏斜。因此，EGT是一种免疫调节剂，也是TLR刺激下的氧化还原控制剂，可诱导M1巨噬细胞和炎症中的Th 17转移。
L-Ergothioneine (EGT) is a naturally-occurring amino acid which is characterized by its antioxidant property; yet, the physiological role of EGT has yet to be established. We investigated the immune-enhancing properties of EGT, and found that it acts as a potentiator of toll-like receptor (TLR) signaling. When mouse bone marrow-derived macrophages (BMDMs) were pretreated with EGT, TLR signal-mediated cytokine production was augmented in BMDMs. The results were reproducible with TLR2, 3, 4 and 7 agonists. In particular, IL-6 and IL-12p40 were elevated further by pretreatment with EGT in BMDMs, suggesting the induction of M1 polarization. In co-culture assay with OT-II CD4+ T cells and splenic F4/80+ macrophages, EGT significantly induced Th17 skewing in CD4+ T cells. Thus, EGT is an immune modifier as well as a redox controller under TLR stimulation that induces M1 macrophages and a Th17 shift in inflammation.