Three-dimensional confocal microscopy of colloids

Three-dimensional confocal microscopy of colloids
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DOI:
10.1364/ao.40.004152
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发表时间:
2001-08-20
期刊:
影响因子:
1.9
通讯作者:
Weitz, DA
Weitz, DA
中科院分区:
工程技术4区
文献类型:
--
作者:
Dinsmore, AD;Weeks, ER;Weitz, DA

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共聚焦显微镜用于胶体凝胶、玻璃和二元流体的研究。我们测量胶体颗粒的三维位置,精度约为50 nm(每个颗粒半径的一小部分),时间分辨率足以同时跟踪数千个颗粒的热运动。这些信息使我们能够以定性的新方式表征这些材料的结构和动力学,例如,通过量化链和颗粒簇的拓扑结构以及通过测量具有高迁移率的颗粒之间的空间相关性。我们描述了我们的实验技术,并描述了测量,补充光散射的结果。(C)2001年美国光学学会。
Confocal microscopy is used in the study of colloidal gels, glasses, and binary fluids. We measure the three-dimensional positions of colloidal particles with a precision of approximately 50 nm (a small fraction of each particle's radius) and with a time resolution sufficient for tracking the thermal motions of several thousand particles at once. This information allows us to characterize the structure and the dynamics of these materials in qualitatively new ways, for example, by quantifying the topology of chains and clusters of particles as well as by measuring the spatial correlations between particles with high mobilities. We describe our experimental technique and describe measurements that complement the results of light scattering. (C) 2001 Optical Society of America.