Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.
Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.
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DOI：
10.4103/1008-682x.146967
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发表时间：
2015-07
影响因子：
2.9
通讯作者：
Zhu CH
中科院分区：
文献类型：
--
作者：
Rao M;Zhao XL;Yang J;Hu SF;Lei H;Xia W;Zhu CH
在这项实验性前瞻性研究中，我们旨在从精子参数、精液血浆生化标志物和氧化应激的角度分析短暂性阴囊高温对男性生殖器官的影响，以评估不同频率的热暴露是否对精子发生造成不同程度的损害。两组志愿者（每组10人）在43℃水浴中加热睾丸10次，每次30分钟：第1组：连续10天；第2组：每3天一次。治疗前及治疗后16周恢复期检测精子参数、附睾及副性腺功能、精液血浆氧化应激及血清性激素。最后，我们发现精子浓度（与基线水平比较最小值时，第1组P = 0.005，第2组P = 0.008，下同）、活力（分别为P = 0.009和0.021）、低渗肿胀测试评分（分别为P = 0.007和0.008）、总顶体酶活性（P =分别为 0.018 和 0.009），精浆丙二醛浓度增加（P 分别为 0.005 和 0.017）。第 2 组精子浓度的下降幅度大于第 1 组（P = 0.031）。我们的结论是，短暂的阴囊高温严重但可逆地对精子发生产生了负面影响，氧化应激可能参与了这一过程。此外，与连续热暴露相比，间歇性热暴露更严重地抑制精子发生。这可能对临床不孕症病因分析和基于热应激的避孕方法设计具有指导意义。
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.