HLA DRB1*0405-DQB1*0401 haplotype is associated with autoimmune pancreatitis in the Japanese population
HLA DRB1*0405-DQB1*0401 haplotype is associated with autoimmune pancreatitis in the Japanese population
复制标题
DOI：
10.1053/gast.2002.33022
复制
发表时间：
2002-05-01
期刊：
影响因子：
29.4
通讯作者：
Kiyosawa, K
中科院分区：
文献类型：
--
作者：
Kawa, S;Ota, M;Kiyosawa, K
背景与目的：自身免疫性胰腺炎是一种以高血清免疫球蛋白G4浓度为特征的特殊疾病。由于一些自身免疫性疾病与主要组织相容性复合体基因的特定等位基因密切相关，我们研究了HLA等位基因与自身免疫性胰腺炎的相关性。研究方法：应用聚合酶链反应-序列特异性引物法和限制性片段长度多态性法分别对40例自身免疫性胰腺炎、43例慢性钙化性胰腺炎和20：1的健康人进行HLA-A、-B、-C、-DR、-DQ基因分型和HLA-DRB 1、-DQB 1、-DPB 1等位基因分型。结果如下：与健康受试者相比，我们发现自身免疫性胰腺炎患者的DR4显著增加，（73% vs. 44%，校正P = 0.01）和DRB 1 * 0405（58% vs. 21%，校正P = 0.000026）和DQ4（58% vs. 26%，校正P 0.001）和DQB 1 * 0401（58% vs. 21%，校正P 0.000017）。自身免疫性胰腺炎的DRB 1 * 0405-DQB 1 * 0401单倍型与任何HLA I类抗原均无显著相关性，而自身免疫性肝炎的B54-DRB 1 * 0405-DQB 1 * 0401单倍型则相反。DRB 1 * 0405和DQB 1 * 0401在自身免疫性胰腺炎患者中的频率显著高于慢性钙化性胰腺炎患者。结论：在日本人群中，DRB 1 * 0405-DQB 1 * 0401单倍型可能与自身免疫性胰腺炎相关。
Background & Aims: Autoimmune pancreatitis is a distinctive disease entity characterized by high serum immunoglobulin G4 concentrations. Because of the close association between some autoimmune diseases and particular alleles of major histocompatibility complex genes, we investigated the association between HLA alleles and autoimmune pancreatitis. Methods: HLA-A, -B, -C, -DR, and -DQ gene typing and HLA-DRB1, -DQB1, and -DPB1 allele typing were performed by the polymerase chain reaction sequence-specific primers method and the restriction fragment length polymorphism method, respectively, in 40 patients with autoimmune pancreatitis, 43 patients with chronic calcifying pancreatitis, and 20:1 healthy subjects. Results: In patients with autoimmune pancreatitis compared with healthy subjects, we found a significant increase in DR4 (73% vs. 44%, corrected P = 0.01) and DRB1*0405 (58% vs. 21%, corrected P = 0.000026) and DQ4 (58% vs. 26%, corrected P 0.001) and DQB1*0401 (58% vs. 21%, corrected P 0.000017). The DRB1*0405-DQB1*0401 haplotype in autoimmune pancreatitis showed no significant association with any HLA class I antigens, in contrast to the B54-DRB1*0405-DQB1*0401 haplotype reported in autoimmune hepatitis. The frequencies of DRB1*0405 and DQB1*0401 were significantly high in patients with autoimmune pancreatitis compared with chronic calcifying pancreatitis. Conclusions: It is probable that DRB1*0405-DQB1*0401 haplotype is associated with autoimmune pancreatitis in the Japanese population.