ALTERED SURFACTANT FUNCTION AND METABOLISM IN RABBITS WITH ACUTE LUNG INJURY
ALTERED SURFACTANT FUNCTION AND METABOLISM IN RABBITS WITH ACUTE LUNG INJURY
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DOI：
10.1152/jappl.1990.69.6.2303
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发表时间：
1990-12-01
影响因子：
3.3
通讯作者：
JOBE, AH
中科院分区：
文献类型：
--
作者：
LEWIS, JF;IKEGAMI, M;JOBE, AH
用N-亚硝基-N-甲基氨基甲酸酯(NNNMU)诱导兔肺损伤，用差速离心法分离肺泡洗涤亚组分(大、小表面活性物质聚集体)，测定饱和磷脂酰胆碱(Sat PC)池大小和磷脂组成。表面活性物质的代谢也用血管内和气管内的放射性标记进行了研究。随着肺损伤的进展，蛋白通透性、气体交换和顺应性显著异常。在损伤高峰时，肺泡冲洗液中较大的Sat PC池大小减小，同时板层小体内血管内和气管内放射性标记的比活度增加。随之而来的是肺泡冲洗液中的小集合池大小明显增加，以及Sat PC分泌到空气空间的增加。来自肺损伤兔的大小聚集性表面活性物质组分的磷脂组成、总磷脂与蛋白质的比率，以及使用早产呼吸的兔模型进行的体内功能研究都是异常的。这些研究描述了NNNMU损伤肺中肺泡灌洗表面活性物质亚组分的定量、定性和功能变化。
Lung injury was induced in rabbits with N-nitroso-N-methylurethane (NNNMU), and saturated phosphatidylcholine (Sat PC) pool sizes and phospholipid compositions were measured in alveolar wash subfractions isolated by differential centrifugation (large and small surfactant aggregates). Surfactant metabolism also was studied using intravascular and intratracheal radiolabels. Protein permeability, gas exchange, and compliance were significantly abnormal as lung injury progressed. At peak injury, there was a decrease in the large aggregate Sat PC pool size in alveolar wash accompanied by increased uptake of Sat PC from the air space and increased specific activity of both intravascular and intratracheal radiolabels in lamellar bodies. This was followed by a marked rise in the small aggregate pool size in the alveolar wash and increased secretion of Sat PC into the air spaces. Phospholipid compositions, total phospholipid-to-protein ratios, and in vivo functional studies using a preterm ventilated rabbit modfel were abnormal for both large and small aggregate surfactant fractions from the lung-injured rabbits. These studies characterize quantitative, qualititative, and functional changes of alveolar wash surfactant subfractions in NNNMU-injured lungs.