The long-term maintenance of cytotoxic T cell memory does not require persistence of antigen.
The long-term maintenance of cytotoxic T cell memory does not require persistence of antigen.
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DOI：
10.1084/jem.179.1.317
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发表时间：
1994-01-01
期刊：
影响因子：
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通讯作者：
Müllbacher A
中科院分区：
文献类型：
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作者：
Müllbacher A
我已经使用了致敏淋巴细胞转移到同基因照射受体，以调查是否持久的抗原是需要在长期维持溶细胞性T细胞记忆流感病毒。用流感病毒（A/WSN）免疫动物，并在17周后用作T细胞的供体或作为致死性照射的受体。年龄匹配的幼稚小鼠作为对照。在T细胞转移后4、8、16和25周的间隔，实验组和对照组用异源病毒（A/JAP）免疫，并在免疫后3和6 d检测脾细胞对流感病毒的裂解活性。感染后3天的裂解活性（记忆性细胞毒性T细胞应答独有的性质）（Effros，R. B.，J. Bennink和P.C.多尔蒂1978. Cell.免疫学36：345。和Hill，A. B.，R. V. Blanden，C. R. Parrish，和A.穆巴赫1992. Immunol.CellBiol.70：259），仅在用记忆T细胞重建的致敏和未处理的辐射受体中观察到。当将初始T细胞转移到辐射致敏或未致敏的受体中时，未观察到第3天的应答。这些观察结果表明，在没有抗原的情况下，对流感病毒的溶细胞T细胞记忆是长期存在的。
I have used the transfer of primed lymphocytes into syngeneic irradiated recipients to investigate whether the persistence of antigen is required in the long-term maintenance of cytolytic T cell memory to influenza virus. Animals were immunized with influenza virus (A/WSN) and used 17 wk later as either donors for T cells or as lethally irradiated recipients. Naive age-matched mice served as controls. At intervals of 4, 8, 16, and 25 wk after T cell transfer, experimental and control groups were immunized with a heterologous virus (A/JAP) and splenocytes tested for lytic activity to influenza virus 3 and 6 d after immunization. Lytic activity 3 d after infection (a property exclusive to a memory cytotoxic T cell response) (Effros, R. B., J. Bennink, and P. C. Doherty. 1978. Cell. Immunol. 36:345.; and Hill, A. B., R. V. Blanden, C. R. Parrish, and A. Mullbacher. 1992. Immunol. Cell Biol. 70:259), was only observed by primed and naive irradiated recipients reconstituted with memory T cells. No day 3 responses were observed when naive T cells were transferred into irradiated primed or unprimed recipients. These observations demonstrate that cytolytic T cell memory to influenza virus is long lived in the absence of antigen.