Therapeutic effect of MIP-1α-recruited dendritic cells on preestablished solid and metastatic tumors
Therapeutic effect of MIP-1α-recruited dendritic cells on preestablished solid and metastatic tumors
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MIP-1α 募集的树突状细胞对预先建立的实体瘤和转移性肿瘤的治疗作用。
DOI：
10.1016/j.canlet.2010.02.009
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发表时间：
2010-09-01
期刊：
影响因子：
9.7
通讯作者：
Zhang, Yanyun
中科院分区：
文献类型：
--
作者：
Cao, Qi;Jin, Yanliang;Zhang, Yanyun
我们以前发现，树突状细胞（DC）前体可以招募到外周血的B6小鼠巨噬细胞炎症蛋白（MIP）-1 α的管理。当用肿瘤细胞裂解物脉冲时，这些MIP-1 α募集的DC可以诱导抗肿瘤保护性免疫。在这项研究中，MIP-1 α募集的DC在用B16肿瘤细胞裂解物脉冲时不能有效抑制预先建立的肿瘤。然而，接种这些表达MACE-1的DC诱导了针对来自B16-MACE-1细胞的预先建立的实体瘤和转移瘤的抗肿瘤免疫。这些MIP-1 α募集的DC表达更高水平的CCR 7，并显示出对次级淋巴组织更显著的趋化反应。因此，它们在诱导细胞毒性T淋巴细胞和抑制肿瘤发展和转移方面上级骨髓来源的DC。本研究建立了一种新的方法，使用MIP-1 α募集的DC转导肿瘤抗原基因治疗预先建立的实体瘤和转移瘤。(C)2010爱思唯尔爱尔兰有限公司版权所有。
We previously found that dendritic cell (DC) precursors could be recruited into the peripheral blood of B6 mice by administration of macrophage inflammatory protein (MIP)-1 alpha. These MIP-1 alpha-recruited DCs could induce anti-tumor protective immunity when pulsed with tumor cell lysate. In this study, MIP-1 alpha-recruited DCs could not effectively suppress preestablished tumor when pulsed with B16 tumor cell lysate. However, inoculation with these DCs expressing MACE-1 induced an anti-tumor immunity against preestablished solid and metastatic tumor from B16-MACE-1 cells. These MIP-1 alpha-recruited DCs expressed higher level of CCR7 and displayed a more significant chemotactic response toward secondary lymphoid tissue. Therefore, they are superior in the induction of cytotoxic T lymphocytes and the inhibition of tumor development and metastasis than bone marrow-derived DCs. This study established a novel approach to the treatment of preestablished solid and metastatic tumors using MIP-1 alpha-recruited DCs transduced with tumor antigen gene. (C) 2010 Elsevier Ireland Ltd. All rights reserved.