Role of tetraspanins CD9 and CD151 in primary melanocyte motility
Role of tetraspanins CD9 and CD151 in primary melanocyte motility
复制标题
DOI：
10.1111/j.0022-202x.2005.23882.x
复制
发表时间：
2005-11-01
影响因子：
6.5
通讯作者：
Peñas, PF
中科院分区：
文献类型：
--
作者：
García-López, MA;Barreiro, O;Peñas, PF
四联蛋白CD9和CD151与多种细胞类型的细胞运动和细胞间粘附有关。在此，我们研究了这些分子在原发性黑素细胞中的亚细胞定位和功能作用。我们发现内源性四跨蛋白优先聚集在黑素细胞同型细胞间接触区域和树突尖端。通过对转染了CD9-和CD151-GFP（绿色荧光蛋白）的黑素细胞使用延时荧光共聚焦显微镜进一步证实了这些观察结果，表明这些蛋白参与了细胞接触和迁移。使用CD9和CD151阻断性单克隆抗体进行的细胞粘附和迁移实验显示，对细胞-细胞外基质粘附无显著影响，而黑素细胞的迁移显著增强。进一步证实了CD9和CD151对黑素细胞迁移能力的调控，通过小干扰RNA寡核苷酸敲低这些四跨蛋白的内源性表达。因此，四联蛋白分子定位于调节原代人黑素细胞迁移能力的运动结构。
Tetraspanins CD9 and CD151 have been implicated in cellular motility and intercellular adhesion in several cellular types. Here, we have studied the subcellular localization and the functional role of these molecules in primary melanocytes. We found that endogenous tetraspanins preferentially clustered in areas of melanocyte homotypic intercellular contacts and at the tips of dendrites. These observations were further confirmed using time-lapse fluorescence confocal microscopy of melanocytes transfected with CD9- and CD151-GFP (green fluorescent protein) constructs, suggesting an involvement of these proteins in cellular contacts and migration. Cell adhesion and migration assays performed using blocking monoclonal antibodies against CD9 and CD151 showed no significant effect on cell-extracellular matrix adhesion, whereas the migration of melanocytes was significantly enhanced. The regulation of the migratory capacity of melanocytes by CD9 and CD151 was further confirmed knocking down the endogenous expression of these tetraspanins with small interference RNA oligonucleotides. Therefore, tetraspanin molecules are localized at motile structures in primary human melanocytes regulating the migratory capacity of these cells.