Epstein-Barr virus infection and bcl-2 proto-oncogene expression. Separate events in the pathogenesis of Hodgkin's disease?
Epstein-Barr virus infection and bcl-2 proto-oncogene expression. Separate events in the pathogenesis of Hodgkin's disease?
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Epstein-Barr病毒感染和bcl-2原癌基因表达。
DOI：
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发表时间：
1993
影响因子：
6
通讯作者：
G. Slavin
中科院分区：
文献类型：
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作者：
G. Khan;R. Gupta;P. Coates;G. Slavin
本研究旨在探讨EB病毒（Epstein-Barr virus，EBV）编码的潜伏膜蛋白（latent membrane protein，LMP）是否诱导霍奇金病（Hodgkin 'sdisease，HD）的Hodgkin和Reed-Sternberg（HRS）细胞表达BCL-2，从而为EBV在HD发病中的作用提供可能的机制。采用EB病毒编码的RNA原位杂交和LMP免疫组织化学方法对53例HD进行了EB病毒检测。在应用一级单克隆抗体之前，使用组织切片的微波处理对石蜡材料上的BCL-2进行免疫染色。EBV定位于HRS细胞16例（30%）。所有EBV编码RNA原位杂交阳性的病例也表达LMP。在16例（30%）HRS细胞中检测到BCL-2表达，但其中只有2例也为EBV阳性。在这两种情况下，只有偶尔的HRS细胞表达BCL-2，而LMP，这是在几乎所有这样的细胞检测。BCL-2染色主要为胞浆型，伴有一些膜型。这些结果表明，BCL-2的表达，可以检测到HRS细胞中的常规处理的HD组织，而EB病毒不诱导BCL-2的表达在HD，BCL-2可能有一个作用的发病机制中的EB病毒阴性的情况下的HD。
The present study was undertaken to investigate whether Epstein-Barr virus-(EBV) encoded latent membrane protein (LMP) induces the expression of BCL-2 in Hodgkin and Reed-Sternberg (HRS) cells of Hodgkin's disease (HD) and thereby provide a possible mechanism for the role of EBV in the pathogenesis of this disease. Fifty-three cases of HD were studied for the presence of EBV using EBV-encoded RNA in situ hybridization and LMP immunohistochemistry. Immunostaining for BCL-2 on paraffin material was performed using microwave treatment of tissue sections before the application of the primary monoclonal antibody. EBV was located in HRS cells in 16 cases (30%). All cases that were EBV-encoded RNA in situ hybridization positive, also expressed LMP. BCL-2 expression in HRS cells was detected in 16 cases (30%), but only two of these were also EBV-positive. In both of these cases, only occasional HRS cells expressed BCL-2, in contrast to LMP, which was detected in nearly all such cells. BCL-2 staining was predominantly cytoplasmic with some membrane pattern. These results demonstrate that BCL-2 expression can be detected in HRS cells in routinely processed HD tissue and that whereas EBV does not induce the expression of BCL-2 in HD, BCL-2 may have a role in the pathogenesis of EBV-negative cases of HD.