Enteroaggregative Escherichia coli: epidemiology, virulence and detection.
Enteroaggregative Escherichia coli: epidemiology, virulence and detection.
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DOI：
10.1099/jmm.0.46930-0
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发表时间：
2007
影响因子：
3
通讯作者：
A. Weintraub
中科院分区：
文献类型：
--
作者：
A. Weintraub
肠聚集性大肠杆菌（EAEC）是腹泻性大肠杆菌（DEC）的一个亚群，在过去十年中，它作为水样腹泻的病因受到越来越多的关注，水样腹泻通常持续存在。EAEC已从世界各地的儿童和成人中分离出来。除了零星病例外，还描述了由东亚共同体引起的腹泻暴发。EAEC的定义是微生物附着在上皮细胞（如HEp-2）上的能力，以一种非常典型的“堆叠砖”模式。尽管已经发表了许多寻找这类DEC特有的特定毒力因子的研究，但仍不清楚为什么EAEC会引起持续腹泻。此外，EAEC的聚集性由于其细胞自身的凝集性给血清分型带来了很多问题。鉴定EAEC的金标准包括分离试剂和使用组织培养（即HEp-2细胞）进行粘附试验。这种分析在大多数情况下是可靠的；然而，“非典型”EAEC的出现已经在一些出版物中进行了描述。此外，HEp-2检测耗时，需要组织培养实验室和训练有素的工作人员。一些分子生物学分析已经被描述，然而，没有显示100%的特异性。
Enteroaggregative Escherichia coli (EAEC) is a subgroup of diarrhoeagenic E. coli (DEC) that during the past decade has received increasing attention as a cause of watery diarrhoea, which is often persistent. EAEC have been isolated from children and adults worldwide. As well as sporadic cases, outbreaks of EAEC-caused diarrhoea have been described. The definition of EAEC is the ability of the micro-organism to adhere to epithelial cells such as HEp-2 in a very characteristic 'stacked-brick' pattern. Although many studies searching for specific virulence factor(s) unique for this category of DEC have been published it is still unknown why the EAEC cause persistent diarrhoea. In addition, the aggregative property of EAEC causes a lot of problems in serotyping due to the cells auto-agglutinating. The gold standard for identification of EAEC includes isolation of the agent and an adherence assay using tissue culture, viz. HEp-2 cells. This assay is in most cases reliable; however, emergence of 'atypical' EAEC has been described in several publications. In addition, the HEp-2 assay is time consuming, demands a tissue culture lab and trained staff. Several molecular biological assays have been described, however, none show 100 % specificity.