Reduced antioxidant capacities in platelets from patients with autoimmune thrombocytopenia purpura (ITP)
Reduced antioxidant capacities in platelets from patients with autoimmune thrombocytopenia purpura (ITP)
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DOI：
10.3109/09537104.2011.610909
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发表时间：
2012-01-01
期刊：
影响因子：
3.3
通讯作者：
Salama, Abdulgabar
中科院分区：
文献类型：
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作者：
Kamhieh-Milz, Julian;Bal, Guerkan;Salama, Abdulgabar
自身免疫性血小板减少性紫癜（ITP）的特点是血小板计数异常低和出血风险。确切的触发事件仍然难以捉摸。氧化应激可能在多种自身免疫性疾病中发挥作用。 ITP 中的血小板与氧化应激之间的直接联系尚未得到解决。使用 2',7'-二氯二氢荧光素二乙酸酯并结合过氧化氢，对处于活动期 (n = 24) 和缓解期 (n = 12) 的 ITP 患者以及 44 名健康对照者的细胞内血小板抗氧化能力 (AOC) 进行分析。通过比色法研究患者和对照组血清谷胱甘肽过氧化物酶 (GPx)、谷胱甘肽还原酶 (GRed) 和过氧化氢酶 (CAT) 的酶活性 (EA)。 ITP患者活动期AOC急剧降低，平均荧光强度值显着升高。与健康对照相比，在活动期和缓解期观察到更高的 GPx 活性 (p
Autoimmune thrombocytopenic purpura (ITP) is characterized by an abnormally low platelet count and bleeding risks. The exact triggering event remains elusive. Oxidative stress may play a role in several autoimmune diseases. A direct link between platelets in ITP and oxidative stress has not yet been addressed. The intracellular platelet antioxidant capacity (AOC) in ITP patients in the active phase (n = 24) and remission (n = 12), and 44 healthy controls were analysed with 2',7'-dichlorodihydrofluorescein diacetate, and in combination with hydrogen peroxide. Enzyme activities (EA) of serum glutathione peroxidase (GPx), glutathione reductase (GRed) and catalase (CAT) were investigated colourimetrically in patients and controls. The AOC of ITP patients in the active phase was drastically reduced, with significantly high mean fluorescence intensity values. Higher GPx activity was observed in both active phase and remission in comparison to healthy controls (p