Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture
Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture
复制标题
使用两性离子聚（羧基甜菜碱甲基丙烯酸酯）和细胞特异性肽对电纺聚（ε-己内酯）垫表面进行功能化，以捕获内皮祖细胞
DOI：
10.1016/j.msec.2012.12.074
复制
发表时间：
2013-04-01
影响因子：
7.9
通讯作者：
Kong, Deling
中科院分区：
文献类型：
--
作者：
Li, Qian;Wang, Zhihong;Kong, Deling
血管移植物实现快速内皮化的一种新方法是通过内皮祖细胞（EPC）特异性抗体、适体或特异性结合EPCs的肽将外周血中的EPCs吸引到移植物上。受这一想法的启发，静电纺丝聚（ε-己内酯）（PCL）垫用两性离子聚（羧基甜菜碱甲基丙烯酸酯）（PCBMA）和噬菌体展示选择的EPC特异性肽TPSLEQRTVYAK（TPS）修饰。我们测试了改性材料的物理和化学性质、细胞相容性和血小板粘附性，并研究了功能化表面捕获EPCs的特异性。结果表明，用两性离子PCBMA和TPS肽修饰的PCL表现出改善的亲水性，而没有改变形态和破坏的垫。此外，改性材料支持血管细胞的粘附和生长，并抵抗血小板粘附。这些表面还特异性地捕获EPCs，而不是骨髓间充质干细胞和人脐静脉内皮细胞。这种表面功能化的PCL移植物可能为设计新的血管移植物提供新的机会。(C)2012 Elsevier B. V.保留所有权利。
A novel approach for vascular grafts to achieve rapid endothelialization is to attract endothelial progenitor cells (EPCs) from peripheral blood onto grafts via EPC-specific antibodies, aptamer, or peptides that specifically bind to EPCs. Inspired by this idea, the electrospun poly(epsilon-caprolactone) (PCL) mats were modified with zwitterionic poly(carboxybetaine methacrylate) (PCBMA) and phage display-selected-EPC-specific peptide, TPSLEQRTVYAK (TPS). We tested the physical and chemical properties, cyto-compatibility, and platelet adhesion of the modified material, and investigated the specificity of the functionalized surface for capturing EPCs. The results indicated that PCL modified with zwitterionic PCBMA and TPS peptide showed improved hydrophilicity without morphology change and damage of the mats. Furthermore, the modified material supported adherence and growth of vascular cells and resisted platelets adhesion. The surfaces also specifically captured EPCs rather than bone marrow mesenchymal stem cells and human umbilical vein endothelial cells. This surface-functionalized PCL graft may offer new opportunities for designing new vascular grafts. (C) 2012 Elsevier B.V. All rights reserved.