The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase.
The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase.
复制标题
硫磺菌硫化叶菌 2-酮-3-脱氧葡萄糖酸激酶的结构。
DOI：
10.1107/s0907444908036111
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Potter JA
中科院分区：
文献类型：
--
作者：
Potter JA
超嗜热古菌Sulfolobus solfataricus最佳生长温度高于353 K，并利用一种不寻常的混杂非磷酸化Entner-Doudoroff途径代谢葡萄糖和半乳糖。 有人提出，部分磷酸化Entner-Doudoroff途径在S. solfataricus，其中2-酮-3-脱氧葡萄糖酸激酶（KDGK）对于葡萄糖和半乳糖代谢都是混杂的。重组变异链球菌在大肠杆菌（Escherichiacoli）中表达了硫磺酵母KDGK蛋白，纯化并在0.1M乙酸钠（pH 4.1）和1.4M NaCl中结晶。 载脂蛋白S的晶体结构。用分子置换法将Solfataricus KDGK的分离度提高到2.0 μ mol/L，并将2-酮基-3-脱氧葡萄糖酸盐（KDGlu）和ATP类似物的三元复合物在2.1 μ mol/L下分离。 该复合物表明，该酶的磷酸化KDGlu和2-酮-3-脱氧半乳糖酸（KDGal）的能力的结构基础是来自一个微妙的重新定位的残基，保守的同源nonpromiscuous激酶。
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner–Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner–Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 Å and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 Å. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.
影响因子：
1.6
作者：
Kim, Seonghun;Lee, Sun Bok
通讯作者：
Lee, Sun Bok