Dietary medium-chain triglycerides prevent chemically induced experimental colitis in rats
Dietary medium-chain triglycerides prevent chemically induced experimental colitis in rats
复制标题
DOI：
10.1016/j.trsl.2009.08.011
复制
发表时间：
2010-03-01
影响因子：
7.8
通讯作者：
Ogiku, Masahito
中科院分区：
文献类型：
--
作者：
Kono, Hiroshi;Fujii, Hideki;Ogiku, Masahito
研究了膳食中链甘油三酯(MCTs)对2，4，6-三硝基苯磺酸(TNBS)诱导的大鼠实验性结肠炎的影响。给雄性Wistar大鼠结肠内注射TNBS，然后喂以含有MCTS或玉米油的液体饲料(AIN93)作为对照。在TNBS灌肠后1周采集血清和组织标本。病理评估结肠炎的严重程度，并检测组织髓过氧化物酶(MPO)活性。此外，采用逆转录聚合酶链式反应和酶联免疫吸附试验分别检测炎性细胞因子和趋化因子的信使RNA(MRNA)和蛋白水平。在另一组实验中，在喂食流质食物一周后，测量了结肠中Toll样受体(TLR)-4的蛋白表达。为了研究MCT对巨噬细胞的影响，将RAW246.7巨噬细胞与白蛋白与MCT或玉米油的主要成分亚油酸共同孵育。然后检测肿瘤坏死因子-α(TNF-α)的产生。饮食中的MCT显著降低结肠中TLR-4的蛋白水平。此外，与亚油酸孵育的RAW264.7细胞相比，MCT孵育的RAW264.7细胞中TLR-4的表达明显减弱。饮食MCT可减弱结肠中IL-1β(IL-1β)、TNF-α和巨噬细胞炎症蛋白-2(MIP-2)的诱导。此外，与对照组相比，饲喂MCT饲料的动物结肠组织中的MPO活性明显减弱。结果，饮食中的MCT显著改善了化学诱导的结肠炎。MCT作为一种抗炎免疫调节营养素，很可能用于炎症性肠病的治疗。《翻译研究》2010；155：131-141)
The effects of dietary medium-chain triglycerides (MCTs) on experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS) were investigated in rats. Male Wistar rats were given an intracolonic injection of TNBS and were then fed liquid diets containing MCTs or corn oil (AIN93) as controls. Serum and tissue samples were collected 1 week after TNBS enema. The severity of colitis was evaluated pathologically, and tissue myeloperoxidase (MPO) activity was measured. Furthermore, messenger RNA (mRNA) and protein levels for inflammatory cytokines and a chemokine were assessed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. In another set of experiments, the protein expression of Toll-like receptor (TLR)-4 in the colon was measured 1 week after feeding of liquid diets. To investigate the effects of MCTs on macrophages, RAW246.7 macrophages were incubated with media containing albumin conjugated with MCT or linoleic acid, which is the major component of corn oil. Then, the production of tumor necrosis factor-alpha (TNF-alpha) was measured. Dietary MCTs blunted significantly the protein levels of TLR-4 in the colon. Furthermore, the expression of TLR-4 was significantly blunted in RAW264.7 cells incubated with MCTs compared with cells incubated with linoleic acid. Induction of interleukin 1 beta (IL-1 beta), TNF-alpha, and macrophage inflammatory protein-2 (MIP-2) in the colon was attenuated by dietary MCT. Furthermore, MPO activities in the colonic tissue were significantly blunted in animals fed the MCT diets compared with those fed the control diets. As a result, dietary MCTs improved chemically induced colitis significantly. MCTs most likely are useful for the therapy of inflammatory bowel disease as an anti-inflammatory immunomodulating nutrient. (Translational Research 2010;155:131-141)