Insect taste receptors relevant to host identification by recognition of secondary metabolite patterns of non-host plants
Insect taste receptors relevant to host identification by recognition of secondary metabolite patterns of non-host plants
复制标题
DOI：
10.1016/j.bbrc.2018.04.014
复制
发表时间：
2018-05-23
影响因子：
3.1
通讯作者：
Sato, Ryoichi
中科院分区：
文献类型：
--
作者：
Kasubuchi, Mayu;Shii, Fumika;Sato, Ryoichi
味觉系统对于昆虫和其他动物的食物识别至关重要。通常认为，昆虫味觉器官中表达的味觉受体(GRs)对于寄主植物的选择是必不可少的。许多行为研究表明，单食性或寡食性鳞翅目昆虫在寄主植物中利用取食引诱剂和取食阻滞剂之间的平衡，并通过味觉器官感知这些平衡来识别寄主植物。然而，味觉识别的分子机制，特别是摄食威慑物质的分子机制，仍有待阐明。为了更好地理解这一机制，我们研究了单食性昆虫Bombyx mori的孤儿GRS，包括Bombyx mori Gr(BmGr)16、BmGr18和BmGr53。利用哺乳动物细胞中的钙成像，我们首先在鳞翅目昆虫中证实了三种可能的苦味GR广泛地对结构不同的取食威慑物质做出反应。虽然这些GR的系统发育距离相当大，但它们对部分重叠的植物次生代谢物的威慑作用做出了反应。这些发现表明，不仅这三个GR，而且大多数被分配给可能的苦味GR的GR正在为在寄主植物识别中发挥作用的威慑受体提供食物。(C)2018 Elsevier Inc.保留所有权利。
The taste sensing system is crucial for food recognition in insects and other animals. It is commonly believed that insect gustatory receptors (Grs) expressed in gustatory organs are indispensable for host plant selection. Many behavioral studies have shown that mono- or oligo-phagous lepidopteran insects use the balance between feeding attractants and feeding deterrents in host plants and that these are sensed by taste organs for host plant recognition. However, the molecular mechanism underlying taste recognition, especially of feeding deterrents, remains to be elucidated. To better understand this mechanism, we studied orphan Grs, including Bombyx mori Gr (BmGr) 16, BmGr18, and BmGr53, from the mono-phagous insect, Bombyx mori. Using Calcium imaging in mammalian cells, we first confirmed in lepidoptera insects that three of the putative bitter Grs widely responded to structurally different feeding deterrents. Although the phylogenetic distance of these Grs was considerable, they responded to partially overlapping deterrents of plant secondary metabolites. These findings suggest that not only these three Grs but also most of the Grs that have been assigned to putative bitter Grs are feeding deterrent receptors that play a role in host plant recognition. (C) 2018 Elsevier Inc. All rights reserved.