Highly Sensitive Detection of Protein Biomarkers with Organic Electrochemical Transistors
Highly Sensitive Detection of Protein Biomarkers with Organic Electrochemical Transistors
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DOI：
10.1002/adma.201703787
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发表时间：
2017-11-06
影响因子：
29.4
通讯作者：
Yan, Feng
中科院分区：
文献类型：
--
作者：
Fu, Ying;Wang, Naixiang;Yan, Feng
蛋白质生物标志物的分析在疾病诊断中具有重要意义。虽然许多简便、低成本的电化学方法已经得到了广泛的研究，但它们在检测生理环境中低浓度的蛋白质生物标志物时不够灵敏。在这里，本研究报告了一种新型的基于有机电化学晶体管的生物传感器，它可以成功地检测超高灵敏度的肿瘤蛋白生物标志物。这些装置是通过检测门电极上的电化学活性来操作的，这取决于用催化纳米探针标记的蛋白质的浓度。这种蛋白质传感器可以特异性地检测癌症生物标记物--人表皮生长因子受体2，其浓度低至10(-14)克毫升(-1)，比之前报道的电化学方法的检测下限低了几个数量级。此外，该设备可以在不同浓度下成功区分乳腺癌细胞和正常细胞。蛋白质传感器的超高灵敏度归因于有机电化学晶体管固有的放大功能。这项工作为开发高灵敏度、低成本的生物传感器在未来临床分析中检测各种蛋白质生物标志物铺平了道路。
The analysis of protein biomarkers is of great importance in the diagnosis of diseases. Although many convenient and low-cost electrochemical approaches have been extensively investigated, they are not sensitive enough in the detection of protein biomarkers with low concentrations in physiological environments. Here, this study reports a novel organic-electrochemical-transistor-based biosensor that can successfully detect cancer protein biomarkers with ultrahigh sensitivity. The devices are operated by detecting electrochemical activity on gate electrodes, which is dependent on the concentrations of proteins labeled with catalytic nanoprobes. The protein sensors can specifically detect a cancer biomarker, human epidermal growth factor receptor 2, down to the concentration of 10(-14) g mL(-1), which is several orders of magnitude lower than the detection limits of previously reported electrochemical approaches. Moreover, the devices can successfully differentiate breast cancer cells from normal cells at various concentrations. The ultrahigh sensitivity of the protein sensors is attributed to the inherent amplification function of the organic electrochemical transistors. This work paves a way for developing highly sensitive and low-cost biosensors for the detection of various protein biomarkers in clinical analysis in the future.