Analysis of sphingomyelin in plasma membrane at single cells using luminol electrochemiluminescence
Analysis of sphingomyelin in plasma membrane at single cells using luminol electrochemiluminescence
复制标题
使用鲁米诺电化学发光分析单细胞质膜中的鞘磷脂
DOI：
10.1039/c5ra24275d
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Jiang, Depeng
中科院分区：
文献类型：
--
作者：
Chen, Yao;Liu, Yanan;Jiang, Depeng
利用鲁米诺电化学发光技术对单细胞细胞膜鞘磷脂进行了分析，通过一系列反应将鞘磷脂膜生成过氧化氢进行发光检测。单细胞分析结果显示，加入酶混合物前后的发光比存在较大偏差，表明细胞异质性较高。
Sphingomyelin in plasma membrane at single cells was analyzed using luminol electrochemiluminescence, which involved serial reactions to generate hydrogen peroxide from membrane sphingomyelin for luminescence detection. A large deviation of the luminescence ratio before and after the introduction of the enzyme mixture was obtained from single cell analysis indicating the high cellular heterogeneity.