MEMBRANE-PROPERTIES AND SYNAPTIC RESPONSES OF RAT STRIATAL NEURONS INVITRO
MEMBRANE-PROPERTIES AND SYNAPTIC RESPONSES OF RAT STRIATAL NEURONS INVITRO
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DOI：
10.1113/jphysiol.1991.sp018850
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发表时间：
1991-11-01
影响因子：
5.5
通讯作者：
NORTH, RA
中科院分区：
文献类型：
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作者：
JIANG, ZG;NORTH, RA
1.从大鼠脑上斜切一个含有纹状体的组织切片，以保留相邻的皮质和苍白球。细胞内记录来自368个神经元，使用传统或紧密密封的配置。在电生理学上可以区分两种类型的神经元。主细胞（96%）具有非常负的静息电位（-89 mV）和静息膜电位（39 M-OMEGA）下的低输入电阻：在超极化（-120 mV下为99 nS）开始后的数十毫秒内，膜电导（-65 mV下为10 nS）增加。次级细胞（4%）具有较小的负静息电位（-60 mV）和较高的输入电阻（静息电位时为117 M-OMEGA）：超极化导致在数百毫秒内产生具有H电流性质的内向电流。电刺激苍白球或内囊可逆向激活大多数主细胞。细胞内生物胞素标记显示主细胞有一个中等大小的索马（10-18 μ m），广泛的树突树密集地镶嵌着刺，在某些情况下，主轴突向苍白球延伸.电刺激胼胝体或外囊诱发去极化突触后电位。这种突触电位被6-氰基-7-硝基喹喔啉-2，3-二酮（CNQX，10 μ M）和DL-2-氨基-5-膦酰基戊酸（APV，30 μ M）的组合可逆地阻断，但不受荷包牡丹碱（30 μ M）和印防己毒素（100 μ M）的影响。潜在的突触电流有一个快速的组件（达到峰值的时间约为4毫秒），其幅度是线性相关的膜电位，这是由CNQX阻断;在CNQX的突触电流有一个较慢的组件（达到峰值的时间约为10毫秒），这表现出典型的N-甲基-D-天冬氨酸（NMDA）受体的电压依赖性。两个电流在-5 mV时反转。5.纹状体内的局灶性电刺激（距离细胞内记录位点100-300 μ m）诱发被CNQX和APV部分阻断（45- 95%）的突触电位：剩余的突触电位被荷包牡丹碱（30 μ M）阻断。荷包牡丹碱敏感性突触电流在氯平衡电位下发生逆转.研究结果证实，大多数新纹状体神经元（主细胞，中型多刺神经元）项目的苍白球和接收突触输入从大脑皮层介导的兴奋性氨基酸通过NMDA和非NMDA受体的作用。这些细胞也接受由GABA介导的来自纹状体内神经元的突触输入。少数细胞（4%）具有不同的电生理特性，也接受两种类型的突触输入，并被认为是中间神经元。
1. A tissue slice containing a section of striatum was cut obliquely from rat brain so as to preserve adjacent cortex and pallidum. Intracellular recordings were made from 368 neurones, using either conventional or tight-seal configurations.2. Two types of neurone were distinguished electrophysiologically. Principal cells (96%) had very negative resting potentials (-89 mV) and a low input resistance at the resting membrane potential (39 M-OMEGA): membrane conductance (10 nS at -65 mV) increased within tens of milliseconds after the onset of hyperpolarization (99 nS at -120 mV). Secondary cells (4 %) had less negative resting potentials (-60 mV) and a higher input resistance (117 M-OMEGA at the resting potential): hyperpolarization caused an inward current to develop over hundreds of milliseconds that had the properties of H-current.3. Most principal cells were activated antidromically by electrical stimulation of the globus pallidus or internal capsule. Intracellular labelling with biocytin showed that principal cells had a medium sized soma (10-18-mu-m), extensive dendritic trees densely studded with spines and, in some cases, a main axon which extended towards the globus pallidus.4. Electrical stimulation of the corpus callosum or external capsule evoked a depolarizing postsynaptic potential. This synaptic potential was reversibly blocked by a combination of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10-mu-m) and DL-2-amino-5-phosphonovaleric acid (APV, 30-mu-M), but was unaffected by bicuculline (30-mu-M) and picrotoxin (100-mu-M). The underlying synaptic current had a fast component (time to peak about 4 ms), the amplitude of which was linearly related to membrane potential and which was blocked by CNQX; in CNQX the synaptic current had a slower component (time to peak about 10 ms) which showed voltage dependence typical of N-methyl-D-aspartate (NMDA) receptors. Both currents reversed at -5 mV.5. Focal electrical stimulation within the striatum (100-300-mu-m from the site of intracellular recording) evoked a synaptic potential that was partially blocked (45-95 %) by CNQX and APV: the remaining synaptic potential was blocked by bicuculline (30-mu-M). The bicuculline-sensitive synaptic current reversed at the chloride equilibrium potential.6. The findings confirm that the majority of neostriatal neurones (principal cells, medium spiny neurones) project to the pallidum and receive synaptic inputs from cerebral cortex mediated by an excitatory amino acid acting through NMDA and non-NMDA receptors. These cells also receive synaptic inputs from intrinsic striatal neurones mediated by GABA. A minority of cells (4 %) have distinct electrophysiological properties, also receive both types of synaptic input, and are presumed to be interneurones.