The CD163 long-range scavenger receptor cysteine-rich repeat: expression, purification and X-ray crystallographic characterization
The CD163 long-range scavenger receptor cysteine-rich repeat: expression, purification and X-ray crystallographic characterization
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CD163 长程清道夫受体富含半胱氨酸重复序列：表达、纯化和 X 射线晶体学表征
DOI：
10.1107/s2053230x18005551
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发表时间：
2018-05-01
影响因子：
0.9
通讯作者：
Zhang, Gaiping
中科院分区：
文献类型：
--
作者：
Li, Rui;Ma, Hongfang;Zhang, Gaiping
清道夫受体(SRs)在多种生理和病理途径中发挥着重要作用。其中CD163是一种多功能内源性受体，具有一个富含半胱氨酸的长距离清道夫受体(SRCR)重复序列。然而，这个长距离SRCR重复序列的结构和功能细节尚未阐明。在本研究中，CD163长程SRCR重复序列在果蝇施耐德2细胞中表达。重组蛋白经金属亲和层析、阳离子交换层析和大小排斥层析纯化后为均一蛋白。用20%PEG4000，0.15M四水酒石酸钾钠溶液(pH 8.5)衍射法获得单晶，衍射角分辨率为3.30埃。作为长程SRCR重复的第一个视角，这项工作为深入理解SRS及其多重功能奠定了结构基础。
Scavenger receptors (SRs) play critical roles in various physiological and pathological pathways. One of them, CD163, is a multifunctional endocytic receptor and is characterized by a long-range scavenger receptor cysteine-rich (SRCR) repeat. However, the structural and functional details of this long-range SRCR repeat have not yet been elucidated. In this study, the CD163 long-range SRCR repeat was expressed in Drosophila Schneider 2 cells. The recombinant protein was homogeneous after purification by metal-affinity, cation-exchange and size-exclusion chromatography. Single crystals were obtained using 20% PEG 4000, 0.15 M potassium sodium tartrate tetrahydrate pH 8.5 and diffracted to 3.30 angstrom resolution. As the first view of a long-range SRCR repeat, this work lays the structural basis for a deep understanding of SRs and their multiple functions.