Chemical forms of aluminum in xylem sap of tea plants (Camellia sinensis L.)
Chemical forms of aluminum in xylem sap of tea plants (Camellia sinensis L.)
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DOI：
10.1016/j.phytochem.2004.08.043
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发表时间：
2004-10-01
期刊：
影响因子：
3.8
通讯作者：
Yokota, H
中科院分区：
文献类型：
--
作者：
Morita, A;Horie, H;Yokota, H
为了鉴定茶树根部向茎部输送铝（Al）的化学形态，采用Al-27核磁共振和F-19核磁共振对木质部液进行了分析，发现所采集的木质部液中铝（Al）的浓度为0.29 mM，是f的2倍，未检出儿茶素，木质部液中苹果酸的浓度高于柠檬酸，而草酸的浓度可以忽略不计。木质部汁液的Al-27核磁共振谱有两个信号，在11ppm时信号较大，在-1.5 ppm时信号较小。前一个信号与柠檬酸铝模型溶液的峰一致，表明木质部液中存在柠檬酸铝配合物。尽管后一个信号在-1.5 ppm被认为表明木质部液中存在Al-F配合物（1.7 ppm），但木质部液的F-19 NMR谱只有一个信号在-122 ppm，表明木质部液中主要的F配合物是F-。这些结果表明，茶树木质部汁液中Al可能以柠檬酸盐配合物的形式移位，而苹果酸铝、草酸铝和Al- f配合物不是主要的Al配合物。(C) 2004 Elsevier Ltd.版权所有。
To identify the chemical forms of aluminum (Al) transported from roots to shoots of tea plants (C sinensis L.), Al-27-nuclear magnetic resonance and F-19 NMR spectroscopy were used to analyze xylem sap.The concentration of Al in collected xylem sap was 0.29 mM, twice as high as that of F. Catechins were not detected in xylem sap. The concentration of malic acid in xylem sap was higher than that of citric acid, whereas the concentration of oxalic acid was negligible.There were two signals in the Al-27 NMR spectra of xylem sap, a larger signal at 11 ppm and a smaller one at -1.5 ppm. The former signal was consistent with the peak for an Al-citrate model solution, suggesting that an Al-citrate complex was present in xylem sap. Although the latter signal at -1.5 ppm was thought to indicate the presence of an Al-F complex (at 1.7 ppm) in xylem sap, there was only one signal at -122 ppm in the F-19 NMR spectrum of xylem sap, indicating that the main F complex in xylem sap was F-.These results indicate that Al might be translocated as a complex with citrate, while Al-malate, Al-oxalate and Al-F complexes are not major Al complexes in xylem sap of tea plants. (C) 2004 Elsevier Ltd. All rights reserved.