Dissecting virus-plant interactions through proteomics approaches.
Dissecting virus-plant interactions through proteomics approaches.
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DOI：
10.2174/157016410793611792
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发表时间：
2010-12-01
影响因子：
0.8
通讯作者：
Nagy PD
中科院分区：
文献类型：
--
作者：
Xu K;Nagy PD
植物病毒利用细胞因子，包括宿主蛋白质、膜和代谢物，在受感染的细胞中复制并建立全身感染。除了传统的遗传、分子、细胞和生物化学方法研究植物-病毒相互作用外，全球和专门的蛋白质组学方法正在成为鉴定在病毒感染中发挥作用的所有宿主蛋白质的有用方法。各种蛋白质组学方法包括测量病毒感染与未感染细胞中的差异蛋白质表达，分析病毒复制酶或其他病毒诱导的复合物中的病毒和宿主蛋白质组分，以及使用蛋白质阵列或酵母双杂交测定法进行蛋白质组范围筛选以鉴定宿主蛋白-病毒蛋白相互作用。本文综述了近年来利用蛋白质组学方法在植物病毒学研究中取得的进展，并重点介绍了一些已鉴定的宿主蛋白在病毒感染过程中的功能。由于全球蛋白质组学方法通常不能确定病毒感染期间已确定的宿主因子的分子机制，因此还应使用遗传学、生物化学、细胞生物学和其他方法进行额外的实验以表征宿主因子的功能。总的来说，不断改进的蛋白质组学方法有望进一步了解植物-病毒相互作用，这可能会导致植物病毒病控制的新策略。
Plant viruses exploit cellular factors, including host proteins, membranes and metabolites, for their replication in infected cells and to establish systemic infections. Besides traditional genetic, molecular, cellular and biochemical methods studying plant-virus interactions, both global and specialized proteomics methods are emerging as useful approaches for the identification of all the host proteins that play roles in virus infections. The various proteomics approaches include measuring differential protein expression in virus infected versus noninfected cells, analysis of viral and host protein components in the viral replicase or other virus-induced complexes, as well as proteome-wide screens to identify host protein - viral protein interactions using protein arrays or yeast two-hybrid assays. In this review, we will discuss the progress made in plant virology using various proteomics methods, and highlight the functions of some of the identified host proteins during viral infections. Since global proteomics approaches do not usually identify the molecular mechanism of the identified host factors during viral infections, additional experiments using genetics, biochemistry, cell biology and other approaches should also be performed to characterize the functions of host factors. Overall, the ever-improving proteomics approaches promise further understanding of plant-virus interactions that will likely result in new strategies for viral disease control in plants.