Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals
Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals
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DOI：
10.1006/bbrc.2000.3781
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发表时间：
2000-11-19
影响因子：
3.1
通讯作者：
Jwa, NS
中科院分区：
文献类型：
--
作者：
Agrawal, GK;Rakwal, R;Jwa, NS
植物进化出的对抗各种生物/非生物胁迫的策略包括诱导编码发病机制相关 (PR) 蛋白的基因，特别是广泛用于胁迫反应研究的 PR 1 类 (PR1) 基因家族。尽管它作为 PR 家族成员非常重要，并且是双子叶植物植物病害/防御中公认的基因标记，但人们对水稻 PR1 基因知之甚少。最近，我们克隆并表征了第一个 OsPR1a（水稻酸性 PR1）基因（Agrawal 等人，(2000) Biochem. Biophys. Res. Commun. 274, 157-165）。在这里，我们报告了水稻基本 PR1 (OsPR1b) 基因的特征，该基因是通过筛选由茉莉酸 (JA) 处理的水稻幼苗叶制备的 cDNA 文库而鉴定的，为水稻 PR1 基因表达提供了详细且有价值的见解。 OsPR1b 的推导氨基酸序列显示与 OsPR1a 蛋白只有 63.1% 的同源性，而 Southern 印迹分析表明 OsPR1b 是一个多基因家族。 JA 诱导的 OsPR1b 基因也被水杨酸 (SA)、脱落酸 (ABA) 和激动素 (KN) 上调。此外，蛋白磷酸酶抑制剂斑蝥素 (CN) 和内索索 (EN) 强烈诱导 OsPR1b 转录。然而，OsPR1b 不具有切割响应性，这与 OsPR1a 的切割诱导性正好相反。这种诱导是光、时间和剂量依赖性的，如使用 JA、CN 和 EN 所证明的，并且被放线菌酮完全抑制，但不被四环素抑制。 SA和ABA分别与JA同时应用，显示200μM SA或ABA几乎完全抑制JA诱导的OsPR1b转录，但100μM浓缩溶液则不然，这表明JA、SA和ABA之间存在潜在的相互作用，而KN在同时应用时显着增强了JA诱导的OsPR1b转录。此外，同时应用星形孢菌素可以增强JA、CN和EN诱导的OsPR1b转录，特别是CN。最后，与OsPR1a基因的比较分析使我们深入了解PR1基因家族的差异调节，同时提出OsPR1基因作为水稻的重要基因标记，在分析植物防御反应方面具有潜在用途。 (C) 2000 年学术出版社。
Strategies evolved by plants to counteract a variety of biotic/abiotic stresses include induction of genes encoding pathogenesis-related (PR) protein, in particular the PR class 1 (PR1) gene family, widely used in stress response studies. In spite of its immense importance as a PR family member, and an accepted gene marker in plant disease/defense in dicots, little is known about rice PR1 genes. Recently, we cloned and characterized the first OsPR1a (rice acidic PR1) gene (Agrawal et at (2000) Biochem. Biophys. Res. Commun. 274, 157-165). Here, we report characterization of a rice basic PR1 (OsPR1b) gene, identified from screening a cDNA library prepared from jasmonic acid (JA)treated rice seedling leaf, providing detailed and valuable insights into rice PR1 gene expression. The deduced amino acid sequence of OsPR1b reveals only 63.1% homology with the OsPR1a protein, whereas Southern blot analyses indicate that OsPR1b is a multigene family. The JA-inducible OsPR1b gene was also up-regulated by salicylic acid (SA), abscisic acid (ABA), and kinetin (KN). Furthermore, protein phosphatase inhibitors, cantharidin (CN) and endothall (EN) strongly induced the OsPR1b transcript. However, OsPR1b was not cut-responsive, diagrammatically opposite to cut inducibility of OsPR1a. This induction was light-, time-, and dose-dependent, as demonstrated by using, JA, CN, and EN, and completely inhibited by cycloheximide, but not by tetracycline. The simultaneous application of SA, and ABA, with JA, respectively, showed almost complete inhibition of the JA-induced OsPR1b transcript by 200 muM SA or ABA, but not by 100 muM concentrated solutions, suggesting a potential interaction among JA, SA, and ABA, whereas KN dramatically enhanced JA-induced OsPR1b transcript upon simultaneous application. Moreover, a simultaneous application of staurosporine enhances JA-, CN-, and EN-induced OsPR1b transcript, in particular with CN,Finally a comparative analysis with the OsPR1a gene gives us insight into the differential regulation of the PR1 gene family, while proposing OsPR1 genes as important gene markers in rice, with potential use(s) in analyzing plant defense responses. (C) 2000 Academic Press.