Morning and evening circadian oscillations in the suprachiasmatic nucleus in vitro
Morning and evening circadian oscillations in the suprachiasmatic nucleus in vitro
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DOI：
10.1038/73943
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发表时间：
2000-04-01
影响因子：
25
通讯作者：
Schwartz, WJ
中科院分区：
文献类型：
--
作者：
Jagota, A;de la Iglesia, HO;Schwartz, WJ
日常的生物节律是由一种与生俱来的计时机制或“生物钟”控制的。在哺乳动物中，视交叉上核(SCN)中的时钟由多个自主的单细胞振荡器组成，但SCN细胞如何相互作用形成具有连贯的代谢和电节奏的组织尚不清楚，这可能解释了动物的昼夜行为。在这里，我们证明了SCN电生理活动的昼夜节律，记录为仓鼠下丘脑冠状切片的单个日间峰值，当切片在水平面上切割时，显示出两个不同的峰值。这两个峰值的性质表明SCN组织的功能组织是由两个振荡成分组成的时钟，这证实了最初源于行为观察的一个想法。
Daily biological rhythms are governed by an innate timekeeping mechanism, or 'circadian clock'. In mammals, a clock in the suprachiasmatic nucleus (SCN) comprises multiple autonomous single-cell oscillators, but it is unclear how SCN cells interact to form a tissue with coherent metabolic and electrical rhythms that might account for circadian animal behaviors. Here we demonstrate that the circadian rhythm of SCN electrophysiological activity, recorded as a single daytime peak in hamster hypothalamic coronal slices, shows two distinct peaks when slices are cut in the horizontal plane. Substantiating an idea initially derived from behavioral observations, the properties of these two peaks indicate functional organization of SCN tissue as a clock with two oscillating components.