Subcellular localization of Mitf in monocytic cells.
Subcellular localization of Mitf in monocytic cells.
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DOI：
10.1007/s00418-010-0703-0
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发表时间：
2010-06
影响因子：
2.3
通讯作者：
Lin, Yi-Ling
中科院分区：
文献类型：
--
作者：
Lu, Ssu-Yi;Wan, Hsiao-Ching;Li, Mengtao;Lin, Yi-Ling
小眼相关转录因子（Mitf）是一种在调节多种细胞系发育中起重要作用的转录因子。Mitf的亚细胞定位是动态的，并与其转录活性相关。在这项研究中，我们研究了影响Mitf在单核细胞谱系中亚细胞定位的因素，因为Mitf在这些细胞中大量存在。我们发现了Mitf外显子1B1b编码的一个结构域，该结构域对Mitf在细胞质和细胞核之间的交换很重要。该结构域的缺失破坏了Mitf向细胞质的穿梭，并导致其保留在细胞核中。M-CSF和RANKL均可诱导Mitf的核易位。我们发现Mitf核转运受到M-CSF/Mitf蛋白表达比例的极大影响。此外，Mitf的核转运也需要细胞附着于固体表面。
Microphthalmia-associated transcription factor (Mitf) is a transcription factor that plays an important role in regulating the development of several cell lineages. The subcellular localization of Mitf is dynamic and is associated with its transcription activity. In this study, we examined factors that affect its subcellular localization in cells derived from the monocytic lineage since Mitf is present abundantly in these cells. We identified a domain encoded by Mitf exon 1B1b to be important for Mitf to commute between the cytoplasm and the nucleus. Deletion of this domain disrupts the shuttling of Mitf to the cytoplasm and results in its retention in the nucleus. M-CSF and RANKL both induce nuclear translocation of Mitf. We showed that Mitf nuclear transport is greatly influenced by ratio of M-CSF/Mitf protein expression. In addition, cell attachment to a solid surface also is needed for the nuclear transport of Mitf.
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影响因子：
4.8
作者：
Mansky, KC;Sankar, U;Ostrowski, MC
通讯作者：
Ostrowski, MC
影响因子：
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