Sponge Spray-Reaching New Dimensions of Direct Sampling and Analysis by MS
Sponge Spray-Reaching New Dimensions of Direct Sampling and Analysis by MS
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DOI：
10.1021/acs.analchem.7b02957
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发表时间：
2017-11-07
影响因子：
7.4
通讯作者：
Kipper, Karin
中科院分区：
文献类型：
--
作者：
Hecht, Max;Evard, Hanno;Kipper, Karin
用于用质谱仪（MS）分析临床样品的样品制备可能是广泛且昂贵的。简化和加快这一进程将是非常有益的。本文报道了海绵喷雾-一种新的采样和直接MS分析方法-正是尝试。它可以直接分析，而无需从干燥的血液，血浆和尿液中制备任何样品。体积吸收微量取样装置的尖端用于收集精确量的样品，并且从同一尖端，电喷雾可以被引导到质谱仪中。我们在这里证明，虽然有显着的基质效应，定量青霉素G，一种常见的抗菌剂，是可能的，在血浆和尿液中，基本上没有样品制备。
Sample preparation for the analysis of clinical samples with the mass spectrometer (MS) can be extensive and expensive. Simplifying and speeding up the process would be very beneficial. This paper reports sponge spray-a novel sampling and direct MS analysis approach-attempting exactly that. It enables direct analysis without any sample preparation from dried blood, plasma, and urine. The tip of a volumetric absorptive microsampling device is used to collect an exact amount of sample and from that same tip an electrospray can be directed into a mass spectrometer. We demonstrate here that, although with significant matrix effects, quantitation of penicillin G, a common antimicrobial, is, possible in plasma and in urine, with essentially no sample preparation.