THE PRSA LIPOPROTEIN IS ESSENTIAL FOR PROTEIN SECRETION IN BACILLUS-SUBTILIS AND SETS A LIMIT FOR HIGH-LEVEL SECRETION
THE PRSA LIPOPROTEIN IS ESSENTIAL FOR PROTEIN SECRETION IN BACILLUS-SUBTILIS AND SETS A LIMIT FOR HIGH-LEVEL SECRETION
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DOI：
10.1111/j.1365-2958.1993.tb01616.x
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发表时间：
1993-05-01
影响因子：
3.6
通讯作者：
SARVAS, M
中科院分区：
文献类型：
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作者：
KONTINEN, VP;SARVAS, M
枯草芽孢杆菌的prsA基因的突变已经表明该基因参与蛋白质分泌，并且其编码细胞分泌机制的新组分。我们现在证明，基因产物是一种膜相关脂蛋白，推测结合到细胞质膜的外面。用插入物插入prsA基因的实验表明，它对生存力是必不可少的。PrsA蛋白的细胞水平显示在具有降低的外蛋白水平的prsA突变体中降低，这与蛋白分泌中的基本功能一致。通过增加B中prsA的拷贝数引入增加量的细胞PrsA蛋白。枯草杆菌。这增强了，从六倍到两倍，分泌α-淀粉酶和蛋白酶的菌株，表达高水平的这些外切酶。这表明PrsA蛋白是分泌机制的限速成分，这一发现具有相当大的生物技术意义。
Mutations of the prsA gene of Bacillus subtilis have indicated that the gene is involved in protein secretion and it encodes a novel component of the cellular secretion machinery. We now demonstrate that the gene product is a membrane-associated lipoprotein, presumably bound to the outer face of the cytoplasmic membrane. Experiments to inactivate the prsA gene with insertions indicated that it is indispensable for viability. The cellular level of PrsA protein was shown to be decreased in prsA mutants with decreased level of exoproteins, consistent with an essential function in protein secretion. An increased amount of cellular PrsA protein was introduced by increasing the copy number of prsA in B. subtilis. This enhanced, from six- to twofold, the secretion of alpha-amylases and a protease in strains, which expressed high levels of these exoenzymes. This suggests that PrsA protein is the rate-limiting component of the secretion machinery, a finding that is of considerable biotechnological interest.