CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line.
CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line.
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DOI：
10.1093/jb/mvp057
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发表时间：
2009-08
影响因子：
2.7
通讯作者：
T. Yamanishi;T. Hatakeyama;K. Yamaguchi;T. Oda
中科院分区：
文献类型：
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作者：
T. Yamanishi;T. Hatakeyama;K. Yamaguchi;T. Oda
我们发现CEL-I是从海洋无脊椎动物海参总科(Cucumaria Echinata)中分离到的一种GalNAc特异性的C型凝集素，它能诱导RAW264.7细胞表达诱导型一氧化氮合酶(INOS)并产生NO。诱导型一氧化氮合酶抑制剂L-NAME可抑制NO的产生，但脂多糖抑制剂多粘菌素B不能抑制NO的产生。牛血清白蛋白(BSA)显著抑制CEL-I诱导的NO生成和FITC标记的CEL-I与RAW264.7细胞的结合。细胞外调节蛋白激酶、c-jun氨基末端蛋白激酶和p38蛋白激酶3种MAPK抑制剂均不同程度地抑制CEL-I诱导的NO生成。CEL-I的热处理导致CEL-I的活性随温度的升高而降低。这些结果表明CEL-I通过蛋白质与细胞间的相互作用而不是通过结合细胞表面的特定碳水化合物链来诱导RAW264.7细胞产生NO。
We found that CEL-I, a GalNAc-specific C-type lectin isolated from the marine invertebrate Holothuroidea (Cucumaria echinata), induces inducible nitric oxide synthase (iNOS) expression and NO production in RAW264.7 cells. The NO production was inhibited by an iNOS inhibitor, L-NAME, but was not by a lipopolysaccharide (LPS) inhibitor, polymyxin B. In the presence of 0.1-M GalNAc, increased NO production by CEL-I-treated RAW264.7 cells was observed rather than the inhibition. Bovine serum albumin (BSA) significantly inhibited the CEL-I-induced NO production as well as the binding of FITC-labelled CEL-I on RAW264.7 cells. Three MAP kinase inhibitors (specific to extra-cellular regulated kinase, c-jun NH(2)-terminal kinase and p38 MAP kinase) inhibited CEL-I-induced NO production with different extents. Heat-treatment of CEL-I resulted in a decreased activity of CEL-I depending on the temperature. These results suggest that CEL-I induces NO production in RAW264.7 cells through the protein-cell interaction rather than the binding to the specific carbohydrate chains on the cell surface.