Adsorption of calf thymus DNA on Au(110) studied by reflection anisotropy spectroscopy.
Adsorption of calf thymus DNA on Au(110) studied by reflection anisotropy spectroscopy.
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通过反射各向异性光谱研究小牛胸腺 DNA 在 Au(110) 上的吸附。
DOI：
10.1021/la062839j
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
P. Weightman
中科院分区：
文献类型：
--
作者：
M. Cuquerella;Caroline I Smith;D. Fernig;C. Edwards;P. Weightman
反射各向异性光谱 (RAS) 用于研究电化学电池中单链 (ss-) 和双链 (ds-) 小牛胸腺 DNA 在 Au(110) 上的吸附。两种类型的 DNA 均在 +0.6 至 -0.4 V 范围内的电极电位下形成有序结构。两种类型的 DNA 在 -0.6 V 时解吸，并可能在较低的负电位下开始解吸。当在 +0.6 V 下吸附时，两种形式都会产生相似的 RAS 信号并通过磷酸基团吸附。随着电位降低，从 ss-DNA 观察到的 RAS 强度增加到从 ds-DNA 观察到的大约两倍，这一结果被解释为由于 ss-DNA 的吸附从涉及磷酸基团的位点到涉及碱基的位点发生变化。
Reflection anisotropy spectroscopy (RAS) was used to investigate the adsorption of single-stranded (ss-) and double-stranded (ds-) calf thymus DNA on Au(110) in an electrochemical cell. Both types of DNA form ordered structures for electrode potentials in the range from +0.6 to -0.4 V. Both types of DNA desorb at -0.6 V and may start desorbing at lower negative potentials. When adsorbed at +0.6 V, both forms give rise to a similar RAS signal and adsorb through the phosphate groups. As the potential is reduced, the RAS intensity observed from ss-DNA increases to roughly twice that observed from ds-DNA, a result that is interpreted as due to a change in the adsorption of the ss-DNA from sites involving the phosphate groups to sites involving the bases.