Tissue distribution of placental leucine aminopeptidase/oxytocinase during mouse pregnancy
Tissue distribution of placental leucine aminopeptidase/oxytocinase during mouse pregnancy
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DOI：
10.1177/002215540405200111
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发表时间：
2004-01-01
影响因子：
3.2
通讯作者：
Mizutani, S
中科院分区：
文献类型：
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作者：
Kobayashi, H;Nomura, S;Mizutani, S
胎盘亮氨酸氨肽酶（P-LAP），也称为催产素酶，是一种负责水解催产素的酶。该酶与胱氨酸氨基肽酶相同。我们使用免疫组织化学 (IHC) 分析检查了正常成年小鼠以及小鼠怀孕期间的母亲和胎儿中 P-LAP 的组织分布。 P-LAP-免疫反应蛋白以细胞和妊娠阶段依赖性方式在各种器官中表达。在肾脏中，P-LAP 位于远端肾小管和集合管中，但不在近端肾小管中。妊娠早期，母体胰腺中的朗格汉斯岛周围 P-LAP 染色呈阳性。这种染色模式发生变化，因此在妊娠中期，外周细胞和内部细胞均呈阳性，最后在妊娠晚期仅内部细胞呈阳性。在胎儿肝脏的造血细胞中，只有巨核细胞表现出P-LAP的强表达。胎盘迷路滋养细胞顶端表面的染色强度随着妊娠而增加。这些数据表明P-LAP存在于多种组织中，并且其存在受到妊娠和胎儿发育的影响。因此，P-LAP可能在非妊娠、妊娠和胎儿小鼠的各种生理过程中发挥重要作用。
Placental leucine aminopeptidase (P-LAP), also called oxytocinase, is an enzyme responsible for hydrolyzing oxytocin. This enzyme is identical to cystine aminopeptidase. We examined the tissue distribution of P-LAP in normal adult mice and also in mothers and fetuses during mouse pregnancy using immunohistochemical (IHC) analysis. P-LAP-immunoreactive protein was expressed in various organs in a cell- and gestational stage-dependent manner. In the kidney, P-LAP was located in distal and collecting tubules but not in proximal tubules. The islet of Langerhans in the maternal pancreas stained positively for P-LAP in the periphery in early gestation. This staining pattern changed so that both the periphery and inner cells were positive during mid-gestation and finally only inner cells were positive in late gestation. Among the hematopoietic cells in the fetal liver, only megakaryocytes showed strong expression of P-LAP. The staining intensity increased with gestation on the apical surface of trophoblasts in the placental labyrinth. These data demonstrate that P-LAP is present in a variety of tissues, and its presence is affected by pregnancy and fetal development. Therefore, P-LAP may play a significant role in various physiological processes in non-pregnant, pregnant, and fetal mice.