Ultrastructural studies on the replication and morphogenesis of Nairobi sheep disease virus, a Nairovirus
Ultrastructural studies on the replication and morphogenesis of Nairobi sheep disease virus, a Nairovirus
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DOI：
10.1007/bf01718249
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发表时间：
1996-01-01
影响因子：
2.7
通讯作者：
DeMartini, JC
中科院分区：
文献类型：
--
作者：
Rwambo, PM;Shaw, MK;DeMartini, JC
内罗毕绵羊病病毒（NSDV）影响绵羊和山羊，引起严重出血性胃肠炎和高死亡率。在感染后的不同时间，通过对143 B和BHK-21细胞的超薄切片进行电子显微镜检查来确定NSDV的复制和形态发生。感染后4 h（p.i.）在143 B细胞中，观察到病毒粒子从光滑膜囊泡的双层膜的腔侧出芽。形态成熟的病毒颗粒是电子致密的，球形和均匀的大小（100 nm直径）和积累在光滑的膜囊泡与高尔基复合体。在BHK-21克隆13细胞中，到8 h p.i. NSDV的形态发生仅限于高尔基复合体的光滑膜囊泡内，未检测到其他部位的病毒出芽。接种后10 h观察到细胞外病毒颗粒，在表达细胞病变效应之前。在感染后24 h观察细胞病变效应。在143 B细胞中，在36 h p.i.在BHK-21细胞中。NSDV在BHK-21细胞和143 B细胞中的形态和形态发生类似于布尼亚病毒科的其他成员。
The Nairovirus Nairobi sheep disease virus (NSDV) affects sheep and goats causing severe hemorrhagic gastroenteritis and high mortality. Replication and morphogenesis of NSDV was determined by electron microscopic examination of ultra-thin sections of 143B and BHK-21 cells at varying times after infection. By 4 h post-infection (p.i.) of 143B cells, virions budding from the luminal side of the bilayer membrane of smooth membrane vesicles were observed. Morphologically mature virus particles were electron-dense, spherical and of uniform size (100 nm diameter) and accumulated in smooth membrane vesicles associated with the Golgi complex. In BHK-21 clone 13 cells, mature virus particles in smooth membrane vesicles were present by 8 h p.i. The morphogenesis of NSDV was restricted to the smooth membrane vesicles of Golgi complex, and budding of virus from other sites was not detected. Extracellular virus particles were observed by 10 h p.i., before expression of cytopathic effects. The cytopathic effects were observed at 24 h p.i. in 143B cells and at 36 h p.i. in BHK-21 cells. The morphology and morphogenesis of NSDV in BHK-21 cells and in 143B cells resembles that of other members of the family Bunyaviridae.