SELECTIVE CLUSTERING OF GLUTAMATE AND GAMMA-AMINOBUTYRIC-ACID RECEPTORS OPPOSITE TERMINALS RELEASING THE CORRESPONDING NEUROTRANSMITTERS
SELECTIVE CLUSTERING OF GLUTAMATE AND GAMMA-AMINOBUTYRIC-ACID RECEPTORS OPPOSITE TERMINALS RELEASING THE CORRESPONDING NEUROTRANSMITTERS
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DOI：
10.1073/pnas.91.26.12373
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发表时间：
1994-12-20
影响因子：
11.1
通讯作者：
BANKER, G
中科院分区：
文献类型：
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作者：
CRAIG, AM;BLACKSTONE, CD;BANKER, G
一些免疫细胞化学和生理学的研究表明，神经递质受体的浓度在突触后的网站上的神经元，但受体分布的全貌还没有出现。特别是，目前还不清楚是否有选择性地定位受体簇释放相应的神经递质的相对终端，通过使用兴奋性谷氨酸受体亚基GluR 1和抑制性A型γ-氨基丁酸（GABA）受体β 2/3亚基的抗体，我们表明，这些不同的受体类型集群在不同的突触后部位培养的大鼠海马神经元。GABAA受体β 2/3亚基聚集在GABA能末端对面的细胞体和树突轴上，而GluR 1主要聚集在树突棘上，并与谷氨酸能突触相关。慢性阻断诱发递质释放并不能阻断突触后部位的受体聚集。这些结果表明，复杂的机制，涉及神经末梢特异性信号，需要允许不同的突触后受体类型集群相反，只有适当的突触前终端。
Several immunocytochemical and physiological studies have demonstrated a concentration of neurotransmitter receptors at postsynaptic sites on neurons, but an overall picture of receptor distribution has not emerged. In particular, it has not been clear whether receptor clusters are selectively localized opposite terminals that release the corresponding neurotransmitter, By using antibodies against the excitatory glutamate receptor subunit GluR1 and the inhibitory type A gamma-aminobutyric acid (GABA) receptor beta 2/3 subunits, we show that these different receptor types cluster at distinct postsynaptic sites on cultured rat hippocampal neurons. The GABAA receptor beta 2/3 subunits clustered on cell bodies and dendritic shafts opposite GABAergic terminals, whereas GluR1 clustered mainly on dendritic spines and was associated with glutamatergic synapses. Chronic blockade of evoked transmitter release did not block receptor clustering at postsynaptic sites. These results suggest that complex mechanisms involving nerve terminal-specific signals are required to allow different postsynaptic receptor types to cluster opposite only appropriate presynaptic terminals.