Induced expression of oryzain α gene encoding a cysteine proteinase under stress conditions
Induced expression of oryzain α gene encoding a cysteine proteinase under stress conditions
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DOI：
10.1007/s10265-007-0080-5
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发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Peng, Youliang
中科院分区：
文献类型：
--
作者：
Fu, Yanping;Zhao, Wensheng;Peng, Youliang
从水稻中克隆了半胱氨酸蛋白酶基因Oryzainα-A。Aichi-Asahi)感染稻瘟病菌的叶片。推导出的水稻α-A蛋白序列与水稻种子萌发中表达的基因--水稻α基因有很高的同源性。稻瘟病菌诱导水稻α-A基因表达，与水稻亲和互作的转录水平甚至高于不亲和互作。创伤、紫外线照射、水杨酸和脱落酸处理也能诱导水稻α-A基因的表达，茉莉酸甲酯不能诱导其表达。讨论了水稻α-A在水稻细胞死亡中的作用。
Oryzain alpha-A, a cysteine proteinase gene was cloned from rice (Oryza sativa L. cv. Aichi-asahi) leaves infected with Magnaporthe grisea. The protein sequence deduced for oryzain alpha-A shares high identity with that of oryzain alpha, a gene expressed in germinating rice seed. Oryzain alpha-A gene expression was induced by the blast fungus, Magnaporthe grisea, and the transcript level was even higher in the compatible interaction with rice than in the incompatible interaction. Expression of oryzain alpha-A was also inducible by wounding, ultraviolet radiation, and treatment with salicylic acid and abscisic acid, with no expression induced by methyl jasmonate. The function of oryzain alpha-A in cell death in rice is discussed.