Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions
Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions
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DOI：
10.1021/acsenergylett.7b00167
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发表时间：
2017-05-01
影响因子：
22
通讯作者：
Schuhmann, Wolfgang
中科院分区：
文献类型：
--
作者：
Ruff, Adrian;Szczesny, Julian;Schuhmann, Wolfgang
我们报道了基于[NiFeSe]氢化酶的H-2氧化生物阳极的制备。该酶是通过一个专门设计的低电位的viologen改性的聚合物，提供基准H-2氧化电流，即使在失活条件下，由于有效的保护对O-2结合viologen诱导的O-2抑制酶的再活化的装置进行电线。此外，紫精改性的聚合物允许聚合物和生物催化剂的电化学共沉积，并由此控制膜厚度。在厚的和薄的反应层中证明了酶的保护和再活化。
We report on the fabrication of bioanodes for H-2 oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H-2 oxidizing currents even under deactivating conditions owing to efficient protection against O-2 combined with a viologen-induced reactivation of the O-2 inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.