Toll-like receptors, long non-coding RNA NEAT1, and RIG-I expression are associated with HBeAg-positive chronic hepatitis B patients in the active phase.
Toll-like receptors, long non-coding RNA NEAT1, and RIG-I expression are associated with HBeAg-positive chronic hepatitis B patients in the active phase.
复制标题
Toll样受体、长链非编码RNA NEAT1和RIG-I表达与HBeAg阳性慢性乙型肝炎活动期患者相关
DOI：
10.1002/jcla.22886
复制
发表时间：
2019
影响因子：
2.7
通讯作者：
Ou Qishui
中科院分区：
文献类型：
--
作者：
Zeng Yongbin;Wu Wennan;Fu Ya;Chen Shanjian;Chen Tianbin;Yang Bin;Ou Qishui
背景天然免疫在宿主-病毒相互作用中起着至关重要的作用，并极大地影响包括HBV感染在内的病毒复制。然而，很少有研究调查TLR，RIG-I和长非编码RNA NEAT 1在慢性HBV感染（CHB）患者临床样本中可能发挥的抗病毒免疫作用及其与免疫应答之间的关系。在这项研究中，我们试图研究活动期HBeAg阳性CHB初治患者中TLR 1 - 10、RIG-I和NEAT 1表达的mRNA表达水平。方法通过qPCR测定活动期CHB患者和健康对照者的TLR 1 - 10、RIG-I和NEAT 1表达水平。结果慢性乙型肝炎活动期患者肝组织中TLR 1、TLR 6表达水平明显低于健康对照组（P < 0. 05），而活动期患者肝组织中TLR 1、TLR 6表达水平明显低于健康对照组（P < 0. 05）。活动期CHB患者TLR 3水平显著高于健康对照组。与健康对照组相比，CHB患者的总NEAT 1表达异常降低。与健康对照组相比，活动期CHB患者的RIG-I水平显著降低。TLR 6和RIG-I的表达与NEAT 1的表达密切相关。结论慢性HBV感染可通过下调TLR 1、TLR 6、NEAT 1的功能表达而改变机体的天然免疫应答。
BackgroundInnate immunity plays a crucial role in host‐virus interactions and greatly influences viral replication including HBV infection. However, few studies have investigated the possible antiviral immune roles played by TLRs, RIG‐I, and long no‐coding RNA NEAT1 in chronic HBV infection (CHB) patients in clinical samples and their relationships among immune responses. In this study, we sought to investigate the mRNA expression levels of TLR1‐10, RIG‐I, and NEAT1 expression in HBeAg‐positive CHB treatment‐naïve patients with the active phase.MethodsThe expression levels of TLR1‐10, RIG‐I, and NEAT1 of CHB patients with the active phase and healthy controls were measured by qPCR. Serum HBV DNA and routine liver biochemistry including ALT, etc were also measured to evaluate the impaired physiological function of the liver affected by CHB.ResultsThe expression levels of TLR1 and TLR6 in CHB with active phase were remarkably lower than that in healthy controls. The levels of TLR3 in CHB patients with active phase were remarkably higher than that in healthy controls. The total NEAT1 expression was abnormally decreased in CHB patients as compared with healthy controls. The levels of RIG‐I were significantly decreased in CHB patients in the active phase when compared to healthy controls. The expression of TLR6 and RIG‐I was closely correlated with NEAT1 expression. TLR6 level was positively correlated with RIG‐I level.ConclusionChronic HBV infection can alter the innate immune response by downregulating functional expression of TLR1, TLR6, NEAT1.