Characterization of a shortened model of diet alternation in female rats: effects of the CB1 receptor antagonist rimonabant on food intake and anxiety-like behavior.
Characterization of a shortened model of diet alternation in female rats: effects of the CB1 receptor antagonist rimonabant on food intake and anxiety-like behavior.
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DOI：
10.1097/fbp.0000000000000059
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发表时间：
2014-10
影响因子：
1.6
通讯作者：
Cottone P
中科院分区：
文献类型：
--
作者：
Blasio A;Rice KC;Sabino V;Cottone P
饮食失调和肥胖在西方社会普遍存在，而且越来越严重。临床前研究的重点是动物模型的发展，这些模型可以模仿在某些形式的饮食失调和肥胖中观察到的食物摄入的不适应模式。本研究旨在描述最近建立的雌性大鼠可口饮食交替模型。为此，雌性大鼠被连续喂食常规饲料（chow / chow）或间歇性喂食常规饲料（2天）和高糖可口饲料（1天）（chow / palatable）。在饮食循环后，大鼠在获得美味饮食或鼠粮期间给予利莫那班（0、0.3、1、3 mg/kg i.p），并评估食物摄入量和体重。最后，用利莫那班（0 - 3 mg/kg, i.p）对大鼠进行预处理，并在退出美味饮食期间在升高加迷宫中进行测试。交替获得美味食物的雌性大鼠的摄入量循环，在获得美味食物时暴饮暴食，而在返回正常饮食后进食不足。利莫那班治疗导致食物/美味大鼠食物吞咽减少和焦虑样行为增加。在节食周期的大鼠中，没有观察到药物治疗对强迫进食美味食物的影响。这项研究的结果表明，停止交替摄入美味饮食会使个体容易受到利莫那班的焦虑效应的影响，并为肥胖患者在利莫那班治疗后出现严重的精神副作用提供了潜在的病因因素。
The prevalence of eating disorders and obesity in Western societies is epidemic and increasing in severity. Preclinical research focused on the development of animal models which can mimic the maladaptive patterns of food intake observed in certain forms of eating disorders and obesity. This study was aimed at characterizing a recently established model of palatable diet alternation in female rats. For this purpose, females rats were fed either continuously with a regular chow diet (Chow/Chow) or intermittently with a regular chow diet for 2 days and a palatable, high-sucrose diet for 1 day (Chow/Palatable). Following diet cycling, rats were administered rimonabant (0, 0.3, 1, 3 mg/kg i.p.) during access to either palatable diet or chow diet and were assessed for food intake and body weight. Finally, rats were pretreated with rimonabant (0 – 3 mg/kg, i.p.) and tested in the elevated plus maze during withdrawal from the palatable diet. Female rats with alternating access to palatable food cycled their intake, overeating during access to the palatable diet, and under-eating upon returning to the regular chow diet. Rimonabant treatment resulted in increased chow hypophagia and anxiety-like behavior in Chow/Palatable rats. No effect of drug treatment was observed on the compulsive eating of palatable food in the diet cycled rats. The results of this study suggest that withdrawal from alternating access to the palatable diet makes individuals vulnerable to the anxiogenic effects of rimonabant and provide etiological factors potentially responsible for the emergence of severe psychiatric side-effects following rimonabant treatment in obese patients.