Bacteria-enhanced dilute acid pretreatment of lignocellulosic biomass.
Bacteria-enhanced dilute acid pretreatment of lignocellulosic biomass.
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DOI：
10.1016/j.biortech.2017.08.037
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发表时间：
2017-12
影响因子：
11.4
通讯作者：
Xu Yan;Zhongren Wang;Kejing Zhang;Mengying Si;Mingren Liu;L. Chai;Xue-duan Liu;Yan Shi
中科院分区：
文献类型：
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作者：
Xu Yan;Zhongren Wang;Kejing Zhang;Mengying Si;Mingren Liu;L. Chai;Xue-duan Liu;Yan Shi
预处理是木质纤维素生物质大规模、低成本生产生物制品不可缺少的环节。本文介绍了一种新的细菌强化稀酸预处理（BE-DAP）策略，采用了一株具有降解木质素潜力的细菌-铜亲和菌B-8。对稻草理化结构的多尺度表征表明，嗜铜菌B-8能作用于稀酸预处理（DAP）过程中形成的木质素液滴，并将其挖出，修复稻草表面的裂纹和孔洞，使稻草表面形成一个开放的多孔结构，便于酶进入内部纤维素。结果表明，经BE-DAP预处理后，RS的酶消化率比未经处理的RS提高了35-70%和173-244%。BE-DAP策略及其物理化学机理为木质纤维素预处理开辟了新的前景。
Pretreatment is indispensable for the large-scale and low-cost bio-products production from lignocellulosic biomass. Herein, a new bacteria-enhanced dilute acid pretreatment (BE-DAP) strategy was introduced.CupriavidusbasilensisB-8 as a potential bacterium for lignin degradation was employed. Multi-scale characterizations on the physicochemical structure of rice straw indicated thatCupriavidus basilensisB-8 could act on the lignin droplets formed in dilute acid pretreatment (DAP), and dig out these droplets to recover cracks and holes on rice straw surface, leaving an opened and porous structure for the easy access of enzyme to inner cellulose. Eventually, the enzymatic digestibility of RS was increased by 35–70% and 173–244% in BE-DAP compared to DAP pretreated and untreated RS, respectively. The BE-DAP strategy, as well as its physicochemical mechanism, opened new perspectives for lignocellulose pretreatment.