Central sympathetic modulation of tissue cytokine response to hemorrhage.
Central sympathetic modulation of tissue cytokine response to hemorrhage.
复制标题
中枢交感神经调节组织细胞因子对出血的反应。
DOI：
10.1159/000026382
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发表时间：
1999
影响因子：
2.4
通讯作者：
Abumrad,NN
中科院分区：
文献类型：
--
作者：
Molina,PE;Abumrad,NN
出血与免疫反应的改变以及促炎细胞因子的循环水平和组织含量的早期增加有关。本研究确定了中枢化学交感神经切除术对固定压力（40 mm Hg）出血后肿瘤坏死因子-α (TNF-α) 和白介素-6 (IL-6) 组织含量早期增加以及阿片类药物和糖皮质激素系统相关激活的影响。意识清醒、不受约束的非肝素化雄性 Sprague-Dawley 大鼠被随机接受脑室内注射 6-羟基多巴胺或等体积的生理盐水 (5 µl)。每组中有一半的动物发生出血，然后用乳酸林格氏溶液进行液体复苏，并在复苏期完成后处死。出血导致脾脏 (30-47%) 和肺 (∼40%) 中 TNF-α 和 IL-6 含量升高。中枢化学交感神经切除术不会改变组织细胞因子含量或β-内啡肽和皮质酮的循环水平。然而，中枢化学交感神经切除术减弱了出血引起的肺和脾脏 TNF-α 的增加，增强了脾脏中 IL-6 的反应，并抑制了循环 β-内啡肽水平的上升。这些结果证明了炎症和阿片类药物对出血应激反应的中枢调节作用。
Hemorrhage is associated with altered immune responses as well as with early increases in circulating levels and tissue content of proinflammatory cytokines. The present study determined the effects of central chemical sympathectomy on the early increase in tissue content of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) following fixed-pressure (40 mm Hg) hemorrhage as well as on the associated activation of the opiate and glucocorticoid systems. Conscious unrestrained nonheparinized male Sprague-Dawley rats were randomized to receive either 6-hydroxydopamine intracerebroventricularly or equal volumes of saline (5 µl). Half of the animals in each group underwent hemorrhage followed by fluid resuscitation with lactated Ringer’s solution, and were sacrificed at completion of the resuscitation period. Hemorrhage elevated TNF-α and IL-6 content in spleen (30–47%) and lung (∼40%). Central chemical sympathectomy did not alter tissue cytokine content or circulating levels of β-endorphin and corticosterone. However, central chemical sympathectomy attenuated the hemorrhage-induced increase in lung and spleen TNF-α, enhanced the IL-6 response in spleen and blunted the rise in circulating β-endorphin levels. These results demonstrate central modulation of the inflammatory and opiate responses to hemorrhagic stress.