DNA-hybridization electron microscopy tertiary structure of 16 S rRNA.
DNA-hybridization electron microscopy tertiary structure of 16 S rRNA.
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DNA 杂交电子显微镜观察 16 S rRNA 的三级结构。
DOI：
10.1016/0022-2836(90)90083-x
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发表时间：
1990
影响因子：
5.6
通讯作者：
Lake,JA
中科院分区：
文献类型：
--
作者：
Oakes,MI;Kahan,L;Lake,JA
用DNA杂交电镜技术在30 S核糖体亚基表面定位了16 S rRNA的7个区域。这些信息已被纳入16 S rRNA的三级结构模型中，约占总16 S rRNA的40%。一个结构标记的平台环提出了一个区域的rRNA的中心域。该结构环绕平台的边缘并且包括区域655-751和769-810。另一个区域，识别复合物，由核苷酸500至545组成，并占据亚基外表面上靠近延伸因子Tu结合位点的区域。已被映射的免疫电子显微镜核糖体蛋白叠加到模型上，以检查可能的相互作用的区域。核糖体蛋白质的模型位置之间的良好相关性，核糖体蛋白质保护的rRNA区域提供了独立的支持这个模型。
Seven regions of 16 S rRNA have been located on the surface of the 30 S ribosomal subunit by DNA-hybridization electron microscopy. This information has been incorporated into a model for the tertiary structure of 16 S rRNA, accounting for approximately 40% of the total 16 S rRNA. A structure labeled the platform ring is proposed for a region of rRNA within the central domain. This structure rings the edges of the platform and includes regions 655–751 and 769–810. Another region, the recognition complex, consists of nucleotides 500 to 545, and occupies a region on the exterior surface of the subunit near the elongation factor Tu binding site. Ribosomal proteins that have been mapped by immunoelectron microscopy are superimposed onto the model in order to examine possible regions of interaction. Good correlation between the model locations of ribosomal proteins, and regions of rRNA protected by ribosomal proteins provide independent support for this model.