Sex differences in mouse heart rate and body temperature and in their regulation by adenosine A1 receptors.
Sex differences in mouse heart rate and body temperature and in their regulation by adenosine A1 receptors.
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小鼠心率和体温及其受腺苷 A1 受体调节的性别差异。
DOI：
10.1111/j.1365-201x.2007.01690.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Fredholm,BB
中科院分区：
文献类型：
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作者：
Yang,J-N;Tiselius,C;Dare,E;Johansson,B;Valen,G;Fredholm,BB
目的：研究清醒状态下腺苷A1受体基因敲除小鼠的心脏功能、体温和运动行为.方法：用遥测装置记录雄性和雌性A1受体（+/+）和（-/-）小鼠在基础条件下和给药后的心率、体温和运动行为.结果：雌性小鼠在白天和晚上都有较高的心率、体温和运动行为.清醒的A1 R（−/−）小鼠心率略有升高，这在雄性中更为明显。Langendorff灌注的失神经A1 R（−/−）心脏的心率也更高。A1 R（−/−）雄性和雌性的体温较高; A1 R（−/−）雌性的自发活动较高，但雄性没有。腺苷受体激动剂R-PIA（0.2 mg kg−1）降低心率和体温，但A1 R（−/−）动物的心率和体温低于A1 R（+/+）小鼠（两个参数均P< 0.001）。 非选择性腺苷受体拮抗剂咖啡因在较低剂量下对心率有轻微的刺激作用，但在75 mg kg−1的剂量下会抑制心率。 在低剂量（7.5 mg kg−1）咖啡因后，两种性别和基因型的体温均升高，而在高剂量（75 mg kg−1）咖啡因后，体温显著降低。 中间剂量的咖啡因30 mg kg− 1会根据基因型和性别增加或降低体温。 运动反应咖啡因是可变的，这取决于基因型和sexy.Conclusion：因此，腺苷A1受体参与调节心率，体温和自发活动，但参与的程度是不同的男性和女性。
Aim:To examine cardiac function, body temperature and locomotor behaviour in the awake adenosine A1receptor knock out mouse of both sexes.Methods:Male and female A1R (+/+) and (−/−) mice, instrumented with telemetric devices, were recorded during basal conditions and after drug administration.Results:Female mice had higher heart rate, body temperature and locomotion, both during daytime and during the night. Awake A1R (−/−) mice had a slightly elevated heart rate, and this was more clear‐cut in males. Heart rate was also higher in Langendorff‐perfused denervated A1R (−/−) hearts. Body temperature was higher in A1R (−/−) males and females; locomotor activity was higher in A1R (−/−) females, but not in males. The adenosine receptor agonist R‐PIA (0.2 mg kg−1) decreased heart rate and body temperature, but less in A1R (−/−) animals than in A1R (+/+) mice (P< 0.001 in both parameters). The unselective adenosine receptor antagonist caffeine had a minor stimulatory effect on heart rate in lower doses, but depressed it at a dose of 75 mg kg−1. Body temperature was increased after a low dose (7.5 mg kg−1) of caffeine in both sexes and genotypes, and markedly reduced after a high dose (75 mg kg−1) of caffeine. An intermediary dose of caffeine 30 mg kg−1increased or decreased body temperature depending on genotype and sex. Locomotor responses to caffeine were variable depending both on genotype and sex.Conclusion:Thus, the adenosine A1receptor is involved in the regulation of heart rate, body temperature and locomotor activity, but the magnitude of the involvement is different in males and females.